Construction of an immunotoxin by linking a monoclonal antibody against the human epidermal growth factor receptor and a hemolytic toxin

dc.creatorAVILA,ANA D
dc.creatorCALDERÓN,CARLOS F
dc.creatorPEREZ,RITA M
dc.creatorPONS,CARMEN
dc.creatorPEREDA,CELIA M
dc.creatorORTIZ,ANA R
dc.date2007-01-01
dc.date.accessioned2017-03-07T16:01:18Z
dc.date.available2017-03-07T16:01:18Z
dc.identifierhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200008
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/394713
dc.descriptionHybrid molecules obtained through conjugation of monoclonal antibodies and toxins constitute an approach under exploration to generate potential agents for the treatment of cancer and other diseases. A frequently employed toxic component in the construction of such immunotoxins is ricin, a plant toxin which inhibits protein synthesis at ribosomal level and so requires to be internalized by the cell. A hemolytic toxin isolated from the sea anemone Stichodactyla helianthus, which is active at the cell membrane level, was linked through a disulfide bond to the anti-epidermal growth factor receptor monoclonal antibody ior egf/r3. The resulting immunotoxin did not exhibit hemolytic activity except under reducing conditions. It was toxic for H125 cells that express the human epidermal growth factor receptor, but non-toxic for U1906 cells that do not express this receptor
dc.formattext/html
dc.languageen
dc.publisherSociedad de Biología de Chile
dc.sourceBiological Research v.40 n.2 2007
dc.subjectEpidermal growth factor
dc.subjectepidermal growth factor receptor
dc.subjecthemolytic toxins
dc.subjectimmunotoxins
dc.subjectmonoclonal antibodies
dc.titleConstruction of an immunotoxin by linking a monoclonal antibody against the human epidermal growth factor receptor and a hemolytic toxin
dc.typeArtículos de revistas


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