Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida

dc.creatorMANCUSO,MONIQUE
dc.creatorAVENDAÑO-HERRERA,RUBÉN
dc.creatorZACCONE,R
dc.creatorTORANZO,ALICIA E
dc.creatorMAGARIÑOS,BEATRIZ
dc.date2007-01-01
dc.date.accessioned2017-03-07T15:55:10Z
dc.date.available2017-03-07T15:55:10Z
dc.identifierhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100009
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/393002
dc.descriptionThis study evaluates the effectiveness of three different molecular techniques, repetitive extragenic palindromic PCR (REP-PCR), enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and the random amplified polymorphic DNA (RAPD-PCR) for rapid typing of Photobacterium damselae ssp. piscicida strains isolated from different species of marine fish and geographic areas. The results obtained by the three methods showed that RAPD and ERIC-PCR were more discriminative for suitable rapid typing of Ph. damselae ssp. piscicida than REP-PCR. The analysis of DNA banding patterns generated by both molecular methods (RAPD and ERIC-PCR) clearly separated the strains into two main groups that strongly correlated with their geographic origin. Moreover, the REP-PCR analysis was less reproducible than the RAPD and ERIC-PCR methods and does not allow the establishment of genetic groups. RAPD and ERIC-PCR constitute valuable tools for molecular typing of Ph. damselae ssp. piscicida strains, which can be used in epidemiological studies of photobacteriosis infections
dc.formattext/html
dc.languageen
dc.publisherSociedad de Biología de Chile
dc.sourceBiological Research v.40 n.1 2007
dc.subjectPhotobacterium damselae ssp
dc.subjectpiscicida
dc.subjectRAPD
dc.subjectERIC-PCR
dc.subjectREP-PCR
dc.titleEvaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida
dc.typeArtículos de revistas


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