Immunosensor based on immobilization of antigenic peptide NS5A-1 from HCV and silk fibroin in nanostructured films
Langmuir, v. 29, n. 11, p. 3829-3834, 2013.
Universidade Estadual Paulista (Unesp)
The peptide NS5A-1 (PPLLESWKDPDYVPPWHG), derived from hepatitis C virus (HCV) NS5A protein, was immobilized into layer-by-layer (LbL) silk fibroin (SF) films. Deposition was monitored by UV-vis absorption measurements at each bilayer deposited. The interaction SF/peptide film induced secondary structure in NS5A-1 as indicated by fluorescence and circular dichroism (CD) measurements. Voltammetric sensor (SF/NS5A-1) properties were observed when the composite film was tested in the presence of anti-HCV. The peptide-silk fibroin interaction studied here showed new architectures for immunosensors based on antigenic peptides and SF as a suitable immobilization matrix. © 2013 American Chemical Society.
Showing items related by title, author, creator and subject.
Antibody derived peptides for detection of Ebola virus glycoprotein RODRIGUEZ MARTINEZ, LUIS MARIO; 256495; RODRIGUEZ MARTINEZ, LUIS MARIO; 256495
Identification of novel peptides of a phage display library with specific monoclonal antibody to the N protein of PRRS virus Villa-Mancera, A.; Reynoso-Palomar, A.; Utrera-Quintana, F.; Córdova-Izquierdo, A.; Olivares-Pérez, J.; Zambrano-González, M.; Trejo-Córdova, A.; Carreón-Luna, L.
Kaposi's sarcoma-associated herpesvirus latency-associated nuclear antigen 1 mimics Epstein-Barr virus EBNA1 immune evasion through central repeat domain effects on protein processing Hyun, Jin Kwun; Da Silva, Suzane Ramos; Shah, Ishita M.; Blake, Neil; Moore, Patrick S.; Chang, Yuan