Assessment of DNA damage by extracts and fractions of Strychnos pseudoquina, a Brazilian medicinal plant with antiulcerogenic activity

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorUniversidade Estadual de Londrina (UEL)
dc.date.accessioned2014-05-27T11:21:57Z
dc.date.accessioned2022-10-05T18:02:45Z
dc.date.available2014-05-27T11:21:57Z
dc.date.available2022-10-05T18:02:45Z
dc.date.created2014-05-27T11:21:57Z
dc.date.issued2006-09-01
dc.identifierFood and Chemical Toxicology, v. 44, n. 9, p. 1585-1589, 2006.
dc.identifier0278-6915
dc.identifierhttp://hdl.handle.net/11449/69054
dc.identifier10.1016/j.fct.2006.03.012
dc.identifier2-s2.0-33745606426
dc.identifier7501930236496670
dc.identifier0000-0003-3032-2556
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3918549
dc.description.abstractStrychnos pseudoquina St. Hil. is a native plant of the Brazilian Savannah, used in popular medicine to treat a number of conditions. Since it contains large quantities of alkaloids with proven antiulcer activity, we tested the genotoxic potential of crude extracts and fractions containing alkaloids and flavonoids from the leaves of this plant, on Salmonella typhimurium and performed the micronucleus test on peripheral blood cells of mice treated in vivo. The results showed that the methanol extract of the leaves of S. pseudoquina is mutagenic to the TA98 (-S9) and TA100 (+S9, -S9) strains of Salmonella. The dichloromethane extract was not mutagenic to any of the tested strains. Fractions enriched with alkaloids or flavonoids were not mutagenic. In vivo tests were done on the crude methanol extract in albino Swiss mice, which were treated, by gavage, with three different doses of the extract. The highest dose tested (1800 mg/kg b.w.) induced micronuclei after acute treatment, confirming the mutagenic potential of the methanol extract of the leaves of S. pseudoquina. In high doses, constituents of S. pseudoquina compounds act on DNA, causing breaks and giving rise to micronuclei in the blood cells of treated animals. © 2006 Elsevier Ltd. All rights reserved.
dc.languageeng
dc.relationFood and Chemical Toxicology
dc.relation3.977
dc.relation1,144
dc.rightsAcesso restrito
dc.sourceScopus
dc.subjectAmes test
dc.subjectMicronucleus
dc.subjectMutagenicity
dc.subjectSalmonella typhimurium
dc.subjectStrychnos pseudoquina
dc.subjectalkaloid
dc.subjectantiulcer agent
dc.subjectdichloromethane
dc.subjectflavonoid
dc.subjectmethanol
dc.subjectplant extract
dc.subjectstrychnos pseudoquina extract
dc.subjectunclassified drug
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectblood cell
dc.subjectbody weight
dc.subjectcontrolled study
dc.subjectDNA damage
dc.subjectDNA strand breakage
dc.subjectdrug megadose
dc.subjectfemale
dc.subjectgenotoxicity
dc.subjectin vivo study
dc.subjectmale
dc.subjectmedicinal plant
dc.subjectmicronucleus
dc.subjectmouse strain
dc.subjectmutagenicity
dc.subjectnonhuman
dc.subjectplant leaf
dc.subjectAdministration, Oral
dc.subjectAnimals
dc.subjectAnti-Ulcer Agents
dc.subjectChemical Fractionation
dc.subjectDose-Response Relationship, Drug
dc.subjectFemale
dc.subjectMale
dc.subjectMethanol
dc.subjectMice
dc.subjectMicronuclei, Chromosome-Defective
dc.subjectMicronucleus Tests
dc.subjectMutagens
dc.subjectPlant Extracts
dc.subjectPlants, Medicinal
dc.subjectReticulocytes
dc.subjectRibosomal Proteins
dc.subjectStrychnos
dc.subjectAnimalia
dc.subjectSalmonella
dc.titleAssessment of DNA damage by extracts and fractions of Strychnos pseudoquina, a Brazilian medicinal plant with antiulcerogenic activity
dc.typeArtigo


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