Artigo
Validação de metodologia analítica por cromatografia líquida de alta eficiência para quantificação de bupivacaína (S75-R25) em nanoesferas de poli(lactídeo-co-glicolídeo)
Fecha
2008-01-01Registro en:
Química Nova. Sociedade Brasileira de Química, v. 31, n. 8, p. 2152-2155, 2008.
0100-4042
10.1590/S0100-40422008000800040
S0100-40422008000800040
WOS:000262580000040
S0100-40422008000800040.pdf
0000-0002-2042-018X
Autor
Universidade Estadual de Campinas (UNICAMP)
Universidade Estadual Paulista (Unesp)
Resumen
Bupivacaine (S75-R25, NovaBupi®) is an amide type local anesthetic widely used. The present work consists of the development and validation of analytical methodology for evaluation of NovaBupi® content in the poly-lactide-co-glycolide nanospheres (PLGA-NS) by high performance liquid chromatography. The separation was made using the reversed-phase column LC-18, acetonitrile/phosphate buffer 85:15 v/v as mobile phase and detection at 220 nm. The results obtained show that the analytical methodology is accurate, reproducible, robust and linear over the concentration range 10-220.0 g/mL of NovaBupi®. The method was applied to determine the encapsulation efficiency and evaluate the release profile of NovaBupi®, showing good results.