Validação de metodologia analítica por cromatografia líquida de alta eficiência para quantificação de bupivacaína (S75-R25) em nanoesferas de poli(lactídeo-co-glicolídeo)

dc.contributorUniversidade Estadual de Campinas (UNICAMP)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:10:24Z
dc.date.accessioned2022-10-05T15:33:10Z
dc.date.available2014-05-20T15:10:24Z
dc.date.available2022-10-05T15:33:10Z
dc.date.created2014-05-20T15:10:24Z
dc.date.issued2008-01-01
dc.identifierQuímica Nova. Sociedade Brasileira de Química, v. 31, n. 8, p. 2152-2155, 2008.
dc.identifier0100-4042
dc.identifierhttp://hdl.handle.net/11449/27614
dc.identifier10.1590/S0100-40422008000800040
dc.identifierS0100-40422008000800040
dc.identifierWOS:000262580000040
dc.identifierS0100-40422008000800040.pdf
dc.identifier0000-0002-2042-018X
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3900523
dc.description.abstractBupivacaine (S75-R25, NovaBupi®) is an amide type local anesthetic widely used. The present work consists of the development and validation of analytical methodology for evaluation of NovaBupi® content in the poly-lactide-co-glycolide nanospheres (PLGA-NS) by high performance liquid chromatography. The separation was made using the reversed-phase column LC-18, acetonitrile/phosphate buffer 85:15 v/v as mobile phase and detection at 220 nm. The results obtained show that the analytical methodology is accurate, reproducible, robust and linear over the concentration range 10-220.0 g/mL of NovaBupi®. The method was applied to determine the encapsulation efficiency and evaluate the release profile of NovaBupi®, showing good results.
dc.languagepor
dc.publisherSociedade Brasileira de Química
dc.relationQuímica Nova
dc.relation0.646
dc.relation0,255
dc.rightsAcesso aberto
dc.sourceSciELO
dc.subjectbupivacaine
dc.subjectPLGA nanospheres
dc.subjectHPLC
dc.titleValidação de metodologia analítica por cromatografia líquida de alta eficiência para quantificação de bupivacaína (S75-R25) em nanoesferas de poli(lactídeo-co-glicolídeo)
dc.typeArtigo


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