| dc.creator | GOMEZ G,CAROLINA | |
| dc.creator | PLESSING R,CARLOS von | |
| dc.creator | GODOY M,C. GLORIA | |
| dc.creator | REINBACH H,ROLANDO | |
| dc.creator | GODOY R,RICARDO | |
| dc.date | 2005-06-01 | |
| dc.date.accessioned | 2017-03-07T15:25:26Z | |
| dc.date.available | 2017-03-07T15:25:26Z | |
| dc.identifier | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072005000200008 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/384726 | |
| dc.description | A practical reversed-phase high-performance liquid chromatography (HPLC) method was developed for 4-chloro-N-furfuryl-5-sulphamoylanthranilic acid (furosemide) determination in plasma. The plasma samples spiked with the drug in presence of propranolol hydrochloride as internal standard, were purified using a liquid - liquid extraction technique. The drugs were separated through a Kromasil 100-5C18 column with a mobile phase composed of acetonitrile and 0.02 M potassium dihydrogen phosphate (34 : 66 v/v) adjusted to pH 3.0 and quantified by fluorescence detection (excitation at 268 nm, emission at 410 nm). The calibration curve was linear ranging between 0.005 and 1.500 mg/mL. The detection limit was 0.001mg/mL and the quantification limit 0.003 mg/mL in plasma. The furosemide recovery from 1mL of plasma was between 99.8 and 101.6 %, with a variation coefficient not higher than 4,85 %. The method is sensitive enough for use in bioavailability and bioequivalence studies after oral administration of 40 mg (a tablet) of furosemide | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad Chilena de Química | |
| dc.source | Journal of the Chilean Chemical Society v.50 n.2 2005 | |
| dc.subject | 4-chloro-N-furfuryl-5-sulphamoylanthranilic acid | |
| dc.subject | urosemide | |
| dc.subject | liquid-liquid extraction from plasma | |
| dc.subject | HPLC with fluorescence detection | |
| dc.subject | validation | |
| dc.title | METHOD VALIDATION FOR THE DETERMINATION OF FUROSEMIDE IN PLASMA BY LIQUID- LIQUID EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION | |
| dc.type | Artículos de revistas | |
