Imaging single-channel calcium microdomains by total internal reflection microscopy

dc.creatorDEMURO,ANGELO
dc.creatorPARKER,IAN
dc.date2004-01-01
dc.date.accessioned2017-03-07T15:24:00Z
dc.date.available2017-03-07T15:24:00Z
dc.identifierhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400025
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/384244
dc.descriptionThe microdomains of Ca2+ in the cytosol around the mouth of open Ca2+ channels are the basic `building blocks' from which cellular Ca2+ signals are constructed. Moreover, the kinetics of local [Ca2+] closely reflect channel gating, so their measurement holds promise as an alternative to electrophysiological patch-clamp recording as a means to study single channel behavior. We have thus explored the development of optical techniques capable of imaging single-channel Ca2+ signals with good spatial and temporal resolution, and describe results obtained using total internal reflection fluorescence microscopy to monitor Ca2+ influx through single N-type channels expressed in Xenopus oocytes
dc.formattext/html
dc.languageen
dc.publisherSociedad de Biología de Chile
dc.sourceBiological Research v.37 n.4 2004
dc.subjectsingle-channel recording
dc.subjectcalcium imaging
dc.subjectTIRFM
dc.subjectN-type Ca2+ channels
dc.titleImaging single-channel calcium microdomains by total internal reflection microscopy
dc.typeArtículos de revistas


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