dc.creator | Schmeda-Hirschmann, G. | |
dc.creator | Rodriguez, J.A. | |
dc.creator | Theoduloz, C. | |
dc.creator | Valderrama, J.A. | |
dc.date | 2012-09-27T20:58:59Z | |
dc.date | 2012-09-27T20:58:59Z | |
dc.date | 2011-03 | |
dc.date.accessioned | 2017-03-07T14:58:33Z | |
dc.date.available | 2017-03-07T14:58:33Z | |
dc.identifier | PLANTA MEDICA Volume: 77 Issue: 4 Pages: 340-345 DOI: 10.1055/s-0030-1250323 | |
dc.identifier | 0032-0943 | |
dc.identifier | http://dspace.utalca.cl/handle/1950/8904 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/375827 | |
dc.description | Schmeda-Hirschmann, G (Schmeda-Hirschmann, Guillermo)1; Rodriguez, JA (Rodriguez, Jaime A.)2; Theoduloz, C (Theoduloz, Cristina)2; ..............1. Univ Talca, Lab Quim Prod Nat, Inst Quim Recursos Nat, Talca, Chile
2. Univ Talca, Depto Bioquim Clin, Fac Ciencias Salud, Talca, Chile | |
dc.description | Semisynthetic aromatic amides from Araucaria araucana diterpene acids have been shown to display a relevant gastroprotective effect with low cytotoxicity. The aim of this work was to assess the gastroprotective effect of amino acid amides from imbricatolic acid and its 8(9)-en isomer in the ethanol/HCl-induced gastric lesions model in mice as well as to determine the cytotoxicity of the obtained compounds on the following human cell lines: normal lung fibroblasts (MRC-5), gastric adenocarcinoma (AGS), and liver hepatocellular carcinoma (Hep G2). The diterpenes 15-acetoxyimbricatolic acid, its 8(9)-en isomer, 15-hydroxyimbricatolic acid, and the 8(9)-en derivative, bearing a COOH function at C-19, were used as starting compounds. New amides with C-protected amino acids were prepared. The study reports the effect of a single oral administration of either compound 50 min before the induction of gastric lesions by ethanol/HCl. Some 20 amino acid monoamides were obtained. Dose-response experiments on the glycyl derivatives showed that at a single oral dose of 100 mg/kg, the compounds presented an effect comparable to the reference drug lansoprazole at 20 mg/kg and at 50 mg/kg reduced gastric lesions by about 50%. All derivatives obtained in amounts >30 mg were compared at a single oral dose of 50 mg/kg. The best gastroprotective effect was observed for the exomethylene derivatives bearing a valine residue at C-19 either with an acetoxy or free hydroxy group at C-15. The tryptophanyl derivative from the acetate belonging to the 8,9-en series presented selective cytotoxicity against hepatocytes. The glycyl amide of 15-acetoxyimbricatolic acid was the most cytotoxic and less selective compound with IC50 values between 47 and 103 mu M for the studied cell lines. This is the first report on the obtention of semisynthetic amino acid amides from labdane diterpenes. | |
dc.language | en | |
dc.subject | amino acid amides | |
dc.subject | Araucaria araucana | |
dc.subject | Araucariaceae | |
dc.subject | cytotoxicity | |
dc.subject | gastroprotective effect | |
dc.subject | labdane diterpenes | |
dc.title | Gastroprotective Effect and Cytotoxicity of Labdeneamides with Amino Acids | |
dc.type | Artículos de revistas | |