dc.creator | Lopes, A. | |
dc.creator | Garcia, J.S. | |
dc.creator | Catharino, R.R. | |
dc.creator | Santos, L.S. | |
dc.creator | Eberlin, M.N. | |
dc.creator | Sarruda, M.A.Z. | |
dc.date | 2008-04-18T21:38:40Z | |
dc.date | 2008-04-18T21:38:40Z | |
dc.date | 2007 | |
dc.date.accessioned | 2017-03-07T14:47:09Z | |
dc.date.available | 2017-03-07T14:47:09Z | |
dc.identifier | Analytica Chimica Acta 590 (2): 166 -172 | |
dc.identifier | 0003-2670 | |
dc.identifier | http://dspace.utalca.cl/handle/1950/4874 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/372720 | |
dc.description | Leonardo Silva Santos. Universidad de Talca, Instituto de Química de Recursos Naturales, P.O. Box 747, Talca, Chile. | |
dc.description | This work describes the optimization of a cloud point extraction (CPE) method for casein proteins from cow milk samples. To promote phase separation, polyoxyethylene(8) isooctylphenyl ether (Triton® X-114) and sodium chloride (NaCl) were used as nonionic surfactant and electrolyte, respectively. Using multivariate studies, four major CPE variables were evaluated: Triton® X-114 concentration, sample volume, NaCl concentration, and pH. The results show that surfactant concentration and sample volume were the main variable affecting the CPE process, with the following optimized parameters: 1% (w/v) Triton® X-114 concentration, 50 μL of sample volume, 6% (w/v) NaCl concentration and extractions carried out at pH 7.0. At these conditions, 923 ± 66 and 67 ± 2 μg mL−1 of total protein were found in the surfactant-rich and surfactant-poor phases, respectively. Finally, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was then used to evaluate those target proteins (s1-casein, s2-casein and β-casein) separation as well as to check the efficiency of the extraction procedure, making a fingerprint of those target proteins possible. | |
dc.format | 2974 bytes | |
dc.format | text/html | |
dc.language | en | |
dc.publisher | Elsevier B.V. | |
dc.subject | Milk; Protein; Cloud point; Extraction; Matrix-assisted laser desorption ionization-time of flight mass spectrometry | |
dc.title | Cloud point extraction applied to casein proteins of cow milk and their identification by mass spectrometry | |
dc.type | Artículos de revistas | |