Razmilic, I.; Schmeda-hirschmann, G.; Reyes, S.; Lopez, I.; Theoduloz, C. 1 Departamento de Ciencias Biológicas, Universidad de Talca, Casilla 747, Talca, Chile.An HPLC method for the simultaneous quantitative determination of rutin and scopoletin in the aerial parts of Fabiana imbricata is presented. The results showed high variability in the scopoletin (240-2,400 mg%) and rutin (195-1,950 mg%) content in the populations surveyed. A micropropagation method for F. imbricata plants was established by culturing shoot tips on Murashige-Skoog (MS) medium containing 1.0 mg/l benzylaminopurine (BAP), 0.01 mg/l naphthaleneacetic acid (NAA), and 0.1 mg/l gibberellic acid (GA3). Auxin addition to the rooting medium, especially 0.5 mg/l indolebutyric acid (IBA) or 0.5 mg/l IBA and 0.1 mg/l NAA, enhances root formation. The micropropagation method presented allowed the obtention of regenerated plantlets in six weeks from shoot tips.