Ceratocystis cacaofunesta genome analysis reveals a large expansion of extracellular phosphatidylinositol-specific phospholipase-C genes (PI-PLC)

dc.contributorUniversidad EAFIT. Departamento de Ciencias
dc.contributorBiodiversidad, Evolución y Conservación
dc.creatorMolano, E.P.L.
dc.creatorCabrera, O.G.
dc.creatorJose, J.
dc.creatordo Nascimento, L.C.
dc.creatorCarazzolle, M.F.
dc.creatorTeixeira, P.J.P.L.
dc.creatorAlvarez, J.C.
dc.creatorTiburcio, R.A.
dc.creatorTokimatu Filho, P.M.
dc.creatorde Lima, G.M.A.
dc.creatorGuido, R.V.C.
dc.creatorCorrêa, T.L.R.
dc.creatorLeme, A.F.P.
dc.creatorMieczkowski, P.
dc.creatorPereira, G.A.G.
dc.date.accessioned2021-03-23T19:52:08Z
dc.date.accessioned2022-09-23T20:59:42Z
dc.date.available2021-03-23T19:52:08Z
dc.date.available2022-09-23T20:59:42Z
dc.date.created2021-03-23T19:52:08Z
dc.date.issued2018-01-17
dc.identifier14712164
dc.identifierWOS;000422884000002
dc.identifierPUBMED;29343217
dc.identifierSCOPUS;2-s2.0-85040746089
dc.identifierhttp://hdl.handle.net/10784/26737
dc.identifier10.1186/s12864-018-4440-4
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3524040
dc.description.abstractBackground: The Ceratocystis genus harbors a large number of phytopathogenic fungi that cause xylem parenchyma degradation and vascular destruction on a broad range of economically important plants. Ceratocystis cacaofunesta is a necrotrophic fungus responsible for lethal wilt disease in cacao. The aim of this work is to analyze the genome of C. cacaofunesta through a comparative approach with genomes of other Sordariomycetes in order to better understand the molecular basis of pathogenicity in the Ceratocystis genus. Results: We present an analysis of the C. cacaofunesta genome focusing on secreted proteins that might constitute pathogenicity factors. Comparative genome analyses among five Ceratocystidaceae species and 23 other Sordariomycetes fungi showed a strong reduction in gene content of the Ceratocystis genus. However, some gene families displayed a remarkable expansion, in particular, the Phosphatidylinositol specific phospholipases-C (PI-PLC) family. Also, evolutionary rate calculations suggest that the evolution process of this family was guided by positive selection. Interestingly, among the 82 PI-PLCs genes identified in the C. cacaofunesta genome, 70 genes encoding extracellular PI-PLCs are grouped in eight small scaffolds surrounded by transposon fragments and scars that could be involved in the rapid evolution of the PI-PLC family. Experimental secretome using LC-MS/MS validated 24% (86 proteins) of the total predicted secretome (342 proteins), including four PI-PLCs and other important pathogenicity factors. Conclusion: Analysis of the Ceratocystis cacaofunesta genome provides evidence that PI-PLCs may play a role in pathogenicity. Subsequent functional studies will be aimed at evaluating this hypothesis. The observed genetic arsenals, together with the analysis of the PI-PLC family shown in this work, reveal significant differences in the Ceratocystis genome compared to the classical vascular fungi, Verticillium and Fusarium. Altogether, our analyses provide new insights into the evolution and the molecular basis of plant pathogenicity. © 2018 The Author(s).
dc.languageeng
dc.publisherBioMed Central Ltd.
dc.relationhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85040746089&doi=10.1186%2fs12864-018-4440-4&partnerID=40&md5=6058b6aedd5783c9f58d4dd67875f57a
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightsAcceso abierto
dc.rightshttps://v2.sherpa.ac.uk/id/publication/issn/1471-2164
dc.sourceBmc Genomics
dc.subjectphosphatidylinositol
dc.subject4,5
dc.subjectbisphosphate
dc.subjectphosphodiesterase
dc.subjectvirulence
dc.subjectfactor
dc.subjectfungal
dc.subjectprotein
dc.subjectphosphatidylinositol
dc.subjectphosphatidylinositol
dc.subject4,5
dc.subjectbisphosphate
dc.subjectphosphodiesterase
dc.subjectArticle
dc.subjectCeratocystis
dc.subjectCeratocystis
dc.subjectcacaofunesta
dc.subjectevolutionary
dc.subjectrate
dc.subjectfungal
dc.subjectgene
dc.subjectfungal
dc.subjectgenome
dc.subjectfungal
dc.subjectvirulence
dc.subjectFusarium
dc.subjectliquid
dc.subjectchromatography-mass
dc.subjectspectrometry
dc.subjectnatural
dc.subjectselection
dc.subjectnonhuman
dc.subjectplant
dc.subjectfungus
dc.subjectinteraction
dc.subjectpositive
dc.subjectselection
dc.subjectprotein
dc.subjecthydrolysis
dc.subjectprotein
dc.subjectsecretion
dc.subjectSordariomycetes
dc.subjecttransposon
dc.subjectVerticillium
dc.subjectAscomycetes
dc.subjectcacao
dc.subjectchemistry
dc.subjectgene
dc.subjectexpression
dc.subjectregulation
dc.subjectgenetics
dc.subjectgenomics
dc.subjectmetabolism
dc.subjectmicrobiology
dc.subjectmolecular
dc.subjectevolution
dc.subjectphylogeny
dc.subjectprocedures
dc.subjectprotein
dc.subjectconformation
dc.subjectAscomycota
dc.subjectCacao
dc.subjectEvolution,
dc.subjectMolecular
dc.subjectFungal
dc.subjectProteins
dc.subjectGene
dc.subjectExpression
dc.subjectRegulation,
dc.subjectPlant
dc.subjectGenome,
dc.subjectFungal
dc.subjectGenomics
dc.subjectPhosphatidylinositols
dc.subjectPhosphoinositide
dc.subjectPhospholipase
dc.subjectC
dc.subjectPhylogeny
dc.subjectProtein
dc.subjectConformation
dc.titleCeratocystis cacaofunesta genome analysis reveals a large expansion of extracellular phosphatidylinositol-specific phospholipase-C genes (PI-PLC)
dc.typepublishedVersion
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typearticle
dc.typeinfo:eu-repo/semantics/article


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