dc.creator | Lozano, José Manuel | |
dc.creator | Espejo, Fabiola | |
dc.creator | Ocampo, Marisol | |
dc.creator | Salazar, Luz Mary | |
dc.creator | Tovar, Diana | |
dc.creator | Barrera, Nubia | |
dc.creator | Guzmán, Fanny | |
dc.creator | Patarroyo, Manuel Elkin | |
dc.date.accessioned | 2020-08-19T14:45:51Z | |
dc.date.accessioned | 2022-09-22T14:41:40Z | |
dc.date.available | 2020-08-19T14:45:51Z | |
dc.date.available | 2022-09-22T14:41:40Z | |
dc.date.created | 2020-08-19T14:45:51Z | |
dc.identifier | ISSN: 1047-8477 | |
dc.identifier | EISSN: 1095-8657 | |
dc.identifier | https://repository.urosario.edu.co/handle/10336/28112 | |
dc.identifier | https://doi.org/10.1016/j.jsb.2004.04.010 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/3441305 | |
dc.description.abstract | Antigen structure modulation represents an approach towards designing subunit malaria vaccines. A specific epitope's ? carbon stereochemistry, as well as its backbone topochemistry, was assessed for obtaining novel malarial immunogens. A variety of MSP-138–61 Plasmodium falciparum epitope pseudopeptides derived were synthesised, based on solid-phase pseudopeptide chemistry strategies; these included all-l, all-d, partially-d substituted, all-?-[NH-CO]-Retro, all-?-[NH-CO]-Retro-inverso, and ?-[CH2NH] reduced amide surrogates. We demonstrate that specific recombinant MSP-134–469 fragment binding to red blood cells (RBCs) is specifically inhibited by non-modified MSP-142–61, as well as by its V52-L53, M51-V52 reduced amide surrogates and partial-d substitutions in K48 and E49. In vivo tests revealed that reduced amide pseudopeptide-immunised Aotus monkeys induced neutralising antibodies specifically recognising the MSP-1 N-terminus region. These findings support the role of molecular conformation in malaria vaccine development. | |
dc.language | eng | |
dc.publisher | Elsevier | |
dc.relation | Journal of Structural Biology, ISSN: 1047-8477;EISSN: 1095-8657, Vol.148, No.1 (2004); pp. 110-122 | |
dc.relation | https://www.sciencedirect.com/science/article/pii/S1047847704001030 | |
dc.relation | 122 | |
dc.relation | No. 1 | |
dc.relation | 110 | |
dc.relation | Journal of Structural Biology | |
dc.relation | Vol. 148 | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.rights | Restringido (Acceso a grupos específicos) | |
dc.source | Journal of Structural Biology | |
dc.source | instname:Universidad del Rosario | |
dc.source | reponame:Repositorio Institucional EdocUR | |
dc.title | Mapping the anatomy of a Plasmodium falciparum MSP-1 epitope using pseudopeptide-induced mono- and polyclonal antibodies and CD and NMR conformation analysis | |
dc.type | article | |