conferenceObject
Vaccination and immunotherapy against bacteria, fungi and parasites (PP-048)
Autor
Lozano J.
Institución
Resumen
We have developed policlonal and monoclonal antibodies directed against specific site-directed peptide modifications named as reduced amide pseudopeptides, all derived from highly conserved malarial antigens from the human’s lethal Plasmodium falciparum parasite. Among these antigens we have chosen the merozoite surface proteins 1 and 2 (MSP-1 and MSP-2). We have thus obtained novel molecular tools with potential applications in the control of malaria. Following Ig in vitro isotype switching, either these antibodies or their derived F(ab)’2 Ig fragments were tested for their ability to suppress the in vivo blood-stage parasitemia through passive immunization in malaria-infected mice. Some of these molecules proved being totally effective in suppressing a lethal malaria blood-stage challenge infection; and others reduced malarial parasitemia. Protection against P. berghei and P. yoelii malaria after Ig passive immunization can be associated with potential novel mechanisms induced by specific site-directed designed pseudopeptides.