Extracción, purificación y modificación de un biopolímero del tipo poli (3-hidroxibutirato) obtenido de la fermentación de ácidos grasos con B. cepacia.

dc.contributorEspinosa Hernández, Armando
dc.contributorBioprocesos y bioprospección
dc.creatorRamos Farfán, Andrés Felipe
dc.date.accessioned2020-03-10T14:01:47Z
dc.date.available2020-03-10T14:01:47Z
dc.date.created2020-03-10T14:01:47Z
dc.date.issued2019-07-19
dc.identifierA.F. Ramos «Extracción, purificación y modificación de un biopolímero del tipo poli (3-hidroxibutirato) obtenido de la fermentación de ácidos grasos con B. cepacia.» Tesis de Maestría en Ingeniería Química, 2020.
dc.identifierhttps://repositorio.unal.edu.co/handle/unal/76043
dc.description.abstractEste trabajo tiene como objetivo estudiar la extracción, purificación y modificación del biopolímero poli(3-hidroxibutirato) producido mediante la fermentación de ácidos grasos con la cepa mutante Burkholderia cepacia B27 con el fin de obtener un proceso novedoso y ambientalmente sostenible para un eventual escalamiento del proceso, y un material con propiedades mecánicas y térmicas que le permitan ser procesado con mayor facilidad y así obtener más salidas en el mercado de los materiales poliméricos. En el trabajo se propone un método novedoso y eficiente para la extracción del polímero usando ácido acético en vez de cloroformo, siendo la nueva metodología más segura para quien la desarrolle debido a la naturaleza de los solventes usados, y ambientalmente más sostenible debido a la significativa reducción de solventes halogenados en el proceso. Por otro lado, se modificó el polímero purificado usando diferentes aditivos, para evaluar el efecto de estos sobre las propiedades mecánicas del material final, y se determinó que las mezclas P3HB/PEG/PLA en diferentes rangos de concentraciones, presentan propiedades mecánicas significativamente mejores a las del P3HB puro que permiten ampliar su espectro de aplicaciones. Este trabajo fue desarrollado con el patrocinio de Colciencias, Biopolab y el Instituto de Biotecnología de la Universidad Nacional, y se desarrolló en el laboratorio de Fermentaciones del IBUN bajo la dirección de Armando Espinosa, Iván Cabeza y Nubia Moreno.
dc.description.abstractThe aim of this work is to study the extraction, purification and modification of the poly(3-hydroxybutyrate) biopolymer produced by the fermentation of fatty acids with the mutant bacteria strain Burkholderia cepacia B27, in order to obtain a novel and environmentally sustainable process for an eventual scaling up, and a material with mechanical and thermal properties that allow it to be processed more easily and thus obtain more oportunities in the market for polymeric materials. The work proposes a new and efficient method for the extraction of the polymer using acetic acid instead of chloroform, being the new methodology safer for the developer due to the nature of the solvents used, and environmentally more sustainable due to the significant reduction of halogenated solvents in the process. On the other hand, the purified polymer was modified using different additives, to evaluate the effect of these on the mechanical properties of the final material, and it was determined that the P3HB/PEG/PLA mixtures in different concentration ranges present significantly better mechanical properties than pure P3HB, which allows to extend its spectrum of applications. This work was developed with the sponsorship of Colciencias, Biopolab and the Institute of Biotechnology of the National University, and was carried out in the Fermentations laboratory of IBUN under the direction of Armando Espinosa, Iván Cabeza and Nubia Moreno.
dc.languagespa
dc.publisherUniversidad Nacional de Colombia - Sede Bogotá
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dc.rightsAtribución-NoComercial 4.0 Internacional
dc.rightsAcceso abierto
dc.rightshttp://creativecommons.org/licenses/by-nc/4.0/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightsDerechos reservados - Universidad Nacional de Colombia
dc.titleExtracción, purificación y modificación de un biopolímero del tipo poli (3-hidroxibutirato) obtenido de la fermentación de ácidos grasos con B. cepacia.
dc.typeOtro


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