dc.contributor | Godoy Silva, Rubén Darío | |
dc.contributor | Grupo de Investigación en Procesos Químicos y Bioquímicos | |
dc.creator | Bello Hernández, Andrés Javier | |
dc.date.accessioned | 2021-08-26T15:58:39Z | |
dc.date.available | 2021-08-26T15:58:39Z | |
dc.date.created | 2021-08-26T15:58:39Z | |
dc.date.issued | 2021-08-20 | |
dc.identifier | https://repositorio.unal.edu.co/handle/unal/80027 | |
dc.identifier | Universidad Nacional de Colombia | |
dc.identifier | Repositorio Institucional Universidad Nacional de Colombia | |
dc.identifier | https://repositorio.unal.edu.co/ | |
dc.description.abstract | Actualmente, los anticuerpos monoclonales (mAbs) corresponden al segmento más importante en el mercado de productos biotecnológicos con efecto terapéutico. La síntesis a escala industrial de este tipo de proteínas recombinantes se lleva a cabo en biorreactores empleando líneas celulares provenientes de un mamífero, las cuales son modificadas genéticamente para incluir dentro de su metabolismo la síntesis del mAb. Para comprender mejor la manera en la que estas células deben ser cultivadas, se realizan ensayos a escala laboratorio, en las cuales se determinan las condiciones más apropiadas para la producción en el biorreactor a escala industrial.
Dentro de los retos más desafiantes en términos técnicos a replicar en reactores a escalas mayores a las del laboratorio se encuentra la velocidad de transferencia de masa de oxígeno. Este gas es vital para el cultivo de las células de mamífero dado su rol en la respiración, por lo tanto, en la obtención de energía para la vida celular. No obstante, dada la baja solubilidad del oxígeno en el agua, es necesario agitar y/o burbujear el medio de cultivo, y así, el gas se solubilizará más rápido en el líquido. La presente tesis de maestría evaluó la cinética del crecimiento, consumo de sustratos y síntesis de coproductos por una línea parental celular de Ovario de Hámster Chino en dos geometrías de cultivo, Erlenmeyer y Spinner. Con el fin de comparar los resultados en las dos configuraciones de biorreactores, se definieron las condiciones de operación (agitación y burbujeo) de manera tal que la velocidad de transferencia de oxígeno fuera igual en las dos geometrías. (Texto tomado de la fuente) | |
dc.description.abstract | Nowadays, monoclonal antibodies (mAbs) correspond to the most important segment in the market for biotechnological products with therapeutic effects. The synthesis of this type of recombinant proteins on an industrial scale is carried out in bioreactors using mammalian cell lines, which are genetically modified to include mAb synthesis within their metabolism. To better understand the way in which these cells should be cultured, tests are carried out on a laboratory scale, in which the conditions for production in the bioreactor on an industrial scale are determined rather.
Assuring an adequate oxygen mass transfer rate is one of the most demanding challenges in technical terms to be replicated in reactors on larger scales than laboratory vessels. This gas is vital for the cultivation of mammalian cells given its role in respiration, and thus, in obtaining energy for cellular life. Nonetheless, since oxygen is sparingly soluble in water, it is necessary to stir and/or bubble the culture medium, for increasing the solubilization rate of the gas in the liquid. In this master's thesis, the kinetics of growth, consumption of substrates and synthesis of co-products by a parental cell line of Chinese Hamster Ovary were evaluated in two culture geometries, Erlenmeyer and Spinner. In order to compare the results in the two bioreactor configurations, the operating conditions (stirring and bubbling) were defined in such a way that the oxygen transfer rate was the same in the two geometries. | |
dc.language | eng | |
dc.publisher | Universidad Nacional de Colombia | |
dc.publisher | Bogotá - Ingeniería - Maestría en Ingeniería - Ingeniería Química | |
dc.publisher | Departamento de Ingeniería Química y Ambiental | |
dc.publisher | Facultad de Ingeniería | |
dc.publisher | Bogotá, Colombia | |
dc.publisher | Universidad Nacional de Colombia - Sede Bogotá | |
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dc.rights | Atribución-NoComercial-CompartirIgual 4.0 Internacional | |
dc.rights | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.rights | Derechos reservados al autor, 2021 | |
dc.title | Evaluation of the volumetric mass transfer coefficient as scale up parameter for CHO
cell cultures for monoclonal antibodies production | |
dc.type | Trabajo de grado - Maestría | |