Aislamiento de mutantes rpoB S531L resistentes a rifampicina en Mycobacterium tuberculosis H37Rv ATCC 25618 e identificación de biomarcadores de adaptación

dc.contributorMurcia Aranguren, Martha Isabel
dc.contributorCarazzone, Chiara
dc.contributorEdgar Orlando Rodríguez Beltrán
dc.contributorMICOBAC-UN
dc.creatorRodríguez Beltrán, Edgar Orlando
dc.date.accessioned2021-06-08T20:55:33Z
dc.date.available2021-06-08T20:55:33Z
dc.date.created2021-06-08T20:55:33Z
dc.date.issued2021
dc.identifierhttps://repositorio.unal.edu.co/handle/unal/79617
dc.identifierUniversidad Nacional de Colombia
dc.identifierRepositorio Institucional Universidad Nacional de Colombia
dc.identifierhttps://repositorio.unal.edu.co/
dc.description.abstractLa TB multirresistente es causada por Mycobacterium tuberculosis (MTB) resistente a rifampicina e isoniazida y constituye un grave problema de salud pública en países emergentes. Puesto que el fitness del agente patógeno es crítico en el desenlace en la infección, el conocimiento sobre el mismo debe ser claro. Trabajos previos sobre resistencia a rifampicina han mostrado situaciones de pérdida y otras de conservación del fitness competitivo. El objetivo del trabajo fue medir la expresión de genes de virulencia / resistencia y los niveles de PDIM relacionándolos con el fitness competitivo de una cepa ATCC25618 RR-TB. Materiales y métodos: Se realizó un ensayo de fitness competitivo usando las cepas MTB ATCC25618 wt: ATCC25618 rpoB-S450L (o S531L) (RifR 1 µg/ml) 1:1 en 50 ml Middlebrook 7H9/OADC (Días 2-12) calculándose tiempos de generación in vitro y fitness relativo. Se extrajeron mRNA y PDIM en días 10/11 y 21 Resultados: Se obtuvo la cepa ATCC 25618 rpoB-S450L (transición en rpoB C1349T). Disminuyó el fitness en rpoB-S450L yH445Y mostrándose fitness dispar interréplicas biológicas. En S450L, hubo dos réplicas con disminución del fitness y una con aumento. Hubo aumento significativo de la expresión de pknG en rpoB-S450L vs silvestre y un aumento no significativo en la cepa silvestre (comparada con rpoB-S450L) en la expresión de ppsA en fase logarítmica que se correlaciona con mayor producción de PDIM, siendo pknG y PDIM potenciales biomarcadores
dc.description.abstractIntroduction. Multidrug-resistant TB, the infection by INH/ RIF-resistant tuberculosis is a severe public health problem in emerging countries. Fitness (W) is a critical component in clinical outcome so it must be explored. Previous works on RIF-resistance have shown some competitive fitness gains and losses. Objective. The objective of these experiments was to measure virulence/ resistance genes and PDIM levels in relation with competitive fitness of a S450L RifR ATCC25618 strain. Materials and methods. We made a competitive fitness in vitro assay with ATCC 25618 wt: ATCC 25618 rpoB-S450L (RifR 1 µg/ml) 1:1 strains in 50 ml Middlebrook OADC and we found generation time (G) in vitro and relative fitness. mRNA and PDIM were extracted on days 10/11 and 21. Results. We obtained ATCC 25618 rpoB S450L mutant (rpoB C1349T transition). Fitness decreased in rpoB-S450L strain with heterogeneous fitness in 3 biological replicas for both S450L and H445Y strains, for the former, 2 replicas with low W and one with high W. There was significant pknG increase in rpoB-S450L and non-significant increase in wt (compared to S450L) in polyketide synthase-ppsA in log phase which is related to increased PDIM, being PDIM and pknG potential biomarkers.
dc.languagespa
dc.publisherUniversidad Nacional de Colombia
dc.publisherBogotá - Ciencias - Maestría en Ciencias - Microbiología
dc.publisherInstituto de Biotecnología (IBUN)
dc.publisherFacultad de Ciencias
dc.publisherBogotá
dc.publisherUniversidad Nacional de Colombia - Sede Bogotá
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dc.rightsAtribución-SinDerivadas 4.0 Internacional
dc.rightshttp://creativecommons.org/licenses/by-nd/4.0/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleAislamiento de mutantes rpoB S531L resistentes a rifampicina en Mycobacterium tuberculosis H37Rv ATCC 25618 e identificación de biomarcadores de adaptación
dc.typeTrabajo de grado - Maestría


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