Estabilidad genética de genotipos de Manihot esculenta sometidos a electroterapia

dc.contributorWenzl, Peter
dc.contributorMuñoz Flórez, Jaime Eduardo
dc.creatorVélez Tobón, Mónica Lorena
dc.date.accessioned2022-08-23T16:06:36Z
dc.date.accessioned2022-09-21T17:21:15Z
dc.date.available2022-08-23T16:06:36Z
dc.date.available2022-09-21T17:21:15Z
dc.date.created2022-08-23T16:06:36Z
dc.date.issued2022
dc.identifierhttps://repositorio.unal.edu.co/handle/unal/82026
dc.identifierUniversidad Nacional de Colombia
dc.identifierRepositorio Institucional Universidad Nacional de Colombia
dc.identifierhttps://repositorio.unal.edu.co/
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3399975
dc.description.abstractLa electroterapia puede usarse como método de limpieza eficaz para eliminación de virus y fitoplasma de yuca in vitro. Sin embargo, se deben tener en cuenta los efectos secundarios de los tratamientos a los que se exponen las plantas para remover patógenos. En este estudio se evaluó la estabilidad genética de materiales sometidos a electroterapia mediante el estudio de los sitios metilados del ADN de 6 genotipos genéticamente diversos de yuca in vitro. Las plantas fueron propagadas clonalmente a partir de una única planta y fueron divididas en dos grupos: materiales que sólo fueron sometidos a subcultivo y materiales que fueron sometidos a electroterapia. Se tomaron muestras de tejido de hojas y raíces antes y después de cada una de estas etapas, se realizó la extracción de ADN genómico y se envió para la secuenciación con la tecnología MS-DArT-Seq (Secuenciación DArT sensible a la metilación) y para el mapeo de las lecturas de los fragmentos generados en el genoma de referencia de M. esculenta. Se identificaron 103607 sitios sensibles a metilación (MSD) distribuidos en todo el genoma. La cantidad e identidad de los sitios metilados fue variable para los genotipos y entre los tratamientos. No se identificaron marcas epigenéticas únicas asociadas con la electroterapia en los materiales. Se concluye que otros factores como el genotipo, el tipo de tejido y el cultivo in vitro podrían estar causando las variaciones de metilación observadas para todas las muestras. (Texto tomado de la fuente)
dc.description.abstractElectrotherapy can be used as an effective cleaning method for elimination of cassava viruses and phytoplasma in vitro. However, the secondary effects of the treatments to which the plants are exposed to remove pathogens must be taken into account. The genetic stability of materials subjected to electrotherapy was evaluated by studying the DNA methylated sites of 6 genetically diverse genotypes of in vitro cassava. The plants were clonally propagated from a single plant and were divided into two groups: materials that were only micropropagated and materials that were subjected to electrotherapy. Leaf and root tissue samples were taken before and after each of these steps, genomic DNA extraction was performed and DNA was sent for sequencing with MS-DArT-Seq technology (Methyl Sensitive DArT Sequencing) and for mapping of the reads of the generated fragments against the reference genome of M. esculenta. 103,607 methylation-sensitive sites (MSD) distributed throughout the genome were identified. The quantity and identity of the methylated sites was variable for genotypes and between treatments. No unique epigenetic marks associated with electrotherapy were identified on the materials. It is concluded that other factors such as genotype, tissue type and in vitro culture could be causing the methylation variations observed for all samples.
dc.languagespa
dc.publisherUniversidad Nacional de Colombia
dc.publisherPalmira - Ciencias Agropecuarias - Maestría en Ciencias Biológicas
dc.publisherFacultad de Ciencias Agropecuarias
dc.publisherPalmira, Colombia
dc.publisherUniversidad Nacional de Colombia - Sede Palmira
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dc.rightsAtribución-NoComercial-SinDerivadas 4.0 Internacional
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.titleEstabilidad genética de genotipos de Manihot esculenta sometidos a electroterapia
dc.typeTesis


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