dc.contributorSerrato Bermúdez, Juan Carlos
dc.contributorBautista Bautista, Eddy Johana
dc.contributorBioprocesos y bioprospección
dc.creatorJaramillo Rodríguez, María Alejandra
dc.date.accessioned2021-01-29T14:27:16Z
dc.date.available2021-01-29T14:27:16Z
dc.date.created2021-01-29T14:27:16Z
dc.date.issued2020-12-07
dc.identifierhttps://repositorio.unal.edu.co/handle/unal/78989
dc.description.abstractLignocellulosic biomass is the most abundant raw material in nature, a part is generated from agro-industrial processes, especially in the agricultural sector. Many of these materials do not have a direct application in industry, therefore their accumulation in large quantities can generate environmental problems. This can be counteracted by using these low-cost materials to generate value-added products such as enzymes, which have an application in different industries. The objective of this work was to design a fermentation process from sugarcane bagasse and wheat bran for the production of hemicellulolytic and cellulolytic enzymes from Trichoderma koningiopsis Th003. Initially, the evaluation of three chemical pretreatment methods was carried out on sugarcane bagasse: basic with 5% NaOH, 2.5% H2SO4 acid, and mixed H2SO4 1% + NaOH 4%. Subsequently, using an experimental design strategy, the physical (fermentation temperature), biological (inoculum concentration), and nutritional (selection and concentration of nitrogen source) conditions were established. Finally, through optimization techniques, the best nutritional conditions (selection of the concentration of carbon and nitrogen sources) and physicochemical (pH, bed height and particle size) for the production of enzymes in a fermentation system were defined. in solid phase. The conditions that favored this production were: temperature of 28 °C; inoculum concentration of 1x106 conidia / mL, bagasse / bran ratio 1: 0.8; yeast extract 3.2 g/L, pH 5.0; particle size 5 cm, and bed height 0.5 cm with five days of fermentation. With them the following enzymatic activities were obtained: FPase 0.275 U/gss, CMCase 1.834 U/gss, and xylanase 1261.05 U/gss.
dc.description.abstractLa biomasa lignocelulósica es muy abundante en la naturaleza, una parte se genera a partir de procesos agroindustriales especialmente del sector agrícola. Muchos de estos materiales no tienen una aplicación directa en la industria, por lo tanto, su acumulación en grandes cantidades puede generar problemas ambientales. Esto se puede contrarrestar mediante el uso de estos materiales de bajo costo para generar productos con valor agregado como enzimas, las cuales tienen una aplicación en diferentes industrias. Este trabajo tuvo como objetivo el diseño de un proceso de fermentación a partir de bagazo de caña y salvado de trigo para la producción de enzimas hemicelulolíticas y celulolíticas de Trichoderma koningiopsis Th003. Inicialmente se realizó la evaluación de tres métodos de pretratamiento químico sobre el bagazo de caña: básico con NaOH 5%, ácido H2SO4 2,5%, y mixto H2SO4 1% + NaOH 4%. Posteriormente, usando una estrategia de diseño experimental, se establecieron las condiciones físicas (temperatura de fermentación), biológicas (concentración del inóculo), nutricionales (selección y concentración de fuente de nitrógeno) del proceso de fermentacíón. Finalmente, a través de técnicas de optimización se definieron las mejores condiciones nutricionales (selección de la concentración de las fuentes de carbono y nitrógeno) y fisicoquímicas (pH, altura de lecho y tamaño de partícula) para la producción de enzimas en un sistema de fermentación en fase sólida. Las condiciones que favorecieron esta producción fueron: temperatura de 28°C; concentración de inóculo de 1x106 conidios/mL, relación bagazo/salvado 1:0,8; extracto de levadura 3,2 g/L, pH 5,0; tamaño de partícula 5 cm, y altura de lecho 0,5 cm con cinco días de fermentación. Con ellas se obtuvieron las siguientes actividades enzimáticas FPasa 0,275 U/gss, CMCasa 1,834 U /gss, y xilanasa 1261,05 U/gss.
dc.languagespa
dc.publisherBogotá - Ingeniería - Maestría en Ingeniería - Ingeniería Química
dc.publisherUniversidad Nacional de Colombia - Sede Bogotá
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dc.rightsAtribución-NoComercial-SinDerivadas 4.0 Internacional
dc.rightsAcceso abierto
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightsDerechos reservados - Universidad Nacional de Colombia
dc.titleDiseño y optimización de un proceso de fermentación para la obtención de enzimas hemicelulolíticas y celulolíticas a partir de Trichoderma koningiopsis Th003
dc.typeOtro


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