Evaluación del efecto de la fuente de carbono sobre el crecimiento y la composición bioquímica de la microalga Parachlorella kessleri

dc.contributorGodoy Silva, Rubén Darío
dc.contributorProcesos Químicos y Bioquímicos
dc.creatorLatorre Hernández, Cindy Carolina
dc.date.accessioned2020-08-21T15:57:20Z
dc.date.available2020-08-21T15:57:20Z
dc.date.created2020-08-21T15:57:20Z
dc.date.issued2020-07-03
dc.identifierhttps://repositorio.unal.edu.co/handle/unal/78153
dc.description.abstractLa microalga Parachlorella kessleri fue sometida a regímenes de cultivo autotróficos, fotoheterotróficos, heterotróficos y mixotróficos, con el fin de evaluar su efecto sobre el crecimiento y la composición bioquímica de la microalga. Las fuentes de carbono orgánico utilizadas fueron glucosa y glicerol, adicionadas en concentraciones de 1, 3 y 5 g·L-1, comparadas siempre con un ensayo control autotrófico sin fuente de carbono orgánica únicamente suplementado con CO2 atmosférico. Los resultados indicaron que P. kessleri tiene mayor afinidad al uso de glucosa, bajo cualquiera de los regímenes de cultivo, que al glicerol, lo cual se vio traducido en mayor concentración final de células en estos tratamientos. La composición bioquímica de la microalga fue afectada por el tipo de régimen de cultivo y por la concentración de fuente de carbono, indicando en términos generales que a medida que se incrementa la concentración de fuente de carbono orgánica se incrementa también la producción de lípidos y carbohidratos. Sin embargo, se observó también que en los tratamientos suplementados con glicerol, a pesar de presentar menores concentraciones celulares máximas, fue en ellos donde se observó una mayor acumulación de carbohidratos y lípidos, sugiriendo un metabolismo destinado más a la acumulación de energía que a la reproducción bajo estas condiciones.
dc.description.abstractThe microalgae Parachlorella kessleri was subjected to autotrophic, photo-heterotrophic, heterotrophic and mixotrophic culture regimes in order to evaluate its growth and biochemical composition. As organic carbon sources, glucose and glycerol were tested in concentrations ranging from 1, 3 and 5 g∙L-1 , while an autotrophic culture was used as a control without organic carbon source added but only atmospheric CO2. Results suggest P. kessleri has a better affinity for glucose than for glycerol under any of the culture regimes, this affinity resulted in higher cell counts for glucose-supplemented cultures. Biochemical composition of the microalgae was affected by culture regime and concentration of organic carbon source; in general, on increase in organic carbon concentration caused on increase in the concentration of lipids and carbohydrates. However, in treatments with glycerol, besides exhibiting lower cellular concentrations, it was observed higher lipid and carbohydrates accumulation, suggesting a metabolism focused to accumulation of energy rather than reproduction under these conditions.
dc.languagespa
dc.publisherBogotá - Ingeniería - Maestría en Ingeniería - Ingeniería Química
dc.publisherUniversidad Nacional de Colombia - Sede Bogotá
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dc.rightsAtribución-NoComercial 4.0 Internacional
dc.rightsAcceso abierto
dc.rightshttp://creativecommons.org/licenses/by-nc/4.0/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightsDerechos reservados - Universidad Nacional de Colombia
dc.titleEvaluación del efecto de la fuente de carbono sobre el crecimiento y la composición bioquímica de la microalga Parachlorella kessleri
dc.typeOtro


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