Efecto de la aplicación de elicitores de origen biótico en la biosíntesis de flavonoides en clavel (Dianthus caryophyllus L) durante la interacción con fusarium oxysporum f sp. dianthi

dc.contributorArdila Barrantes, Harold Duban
dc.contributorCoy Barrera, Ericsson David
dc.contributorEstudio de Actividades Metabolicas Vegetales
dc.creatorRomero Rincón, Ana Elizabeth
dc.date.accessioned2020-08-29T04:22:40Z
dc.date.available2020-08-29T04:22:40Z
dc.date.created2020-08-29T04:22:40Z
dc.date.issued2020-02-14
dc.identifierhttps://repositorio.unal.edu.co/handle/unal/78330
dc.description.abstractFlavonoids are compounds that have multiple functions in plant biochemistry, physiology and ecology, within which defense and protection against stress are described. For carnation flowers, flavonoids have been described as fundamental compounds in defense processes, mainly against Fusarium oxysporum sp. dianthi. This is a vascular pathogen that causes significant losses in the cultivation of this type of flower. In this work, the biosynthesis of flavonoids in carnation roots during the interaction with Fod was studied in detail, evaluating both the constitutive level and the effect of inoculation with the pathogen, also, the compounds present at the constitutive level and some biochemical and molecular parameters associated with its biosynthesis. Additionally, the effect of the application of a potential biotic inducer of resistance prior to inoculation that is obtained directly from the pathogen was evaluated. For this, two commercial varieties were used, which were subjected to an inoculation process and sampling at different hours post-inoculation, to subsequently obtain methanolic extracts and analyze the flavonoids present using HPLC/MS. For biochemical and molecular parameters, spectrophotometric methods and the RT-qPCR technique were used to evaluate the transcriptional levels of some of the enzymes involved. The results presented in this study confirmed the importance of flavonoid biosynthesis in disease resistance. At the same time, it was evidenced that the elicitation prior to the inoculation process stimulated this process in the susceptible variety, which allowed it to be prepared for the battle against the pathogen. The knowledge generated in this study complements the work developed in this model carnation-fusarium, and acredited the central role of flavonoids, especially flavanol-type glycosylates, in inducing resistance using elicitors of biotic origin.
dc.description.abstractLos flavonoides son compuestos que tienen múltiples funciones en la bioquímica, fisiología y ecología de las plantas, dentro de las cuales se describen la defensa y protección frente al estrés. En clavel, los flavonoides han sido descritos como compuestos fundamentales en procesos de defensa, principalmente contra Fusarium oxysporum sp. dianthi; este es un patógeno vascular que causa pérdidas significativas en el cultivo de esta flor. En el presente estudio, se profundizó en la biosíntesis de flavonoides en raíces de clavel durante la interacción con Fod, evaluando tanto a nivel constitutivo, como por efecto de la inoculación con el patógeno, los compuestos presentes a este nivel y algunos parámetros bioquímicos y moleculares asociados a su biosíntesis. Así mismo se evaluó el efecto que tiene la aplicación previa a la inoculación de un potencial inductor de resistencia de origen biótico obtenido directamente del patógeno. Para ello, se emplearon dos variedades comerciales contrastantes a la enfermedad clavel (resistente y susceptible a Fod), que fueron sometidas a un proceso de inoculación y muestreos a diferentes horas posinoculación, para posteriormente obtener extractos metanólicos y analizar los flavonoides presentes usando HPLC/MS. Para los parámetros bioquímicos y moleculares se usaron métodos espectrofotométricos y la técnica RT-qPCR para la evaluación de los niveles transcripcionales de algunas de las enzimas involucradas. Los resultados presentados en este estudio confirmaron la importancia de la biosíntesis de los flavonoides en la resistencia a la enfermedad. Así mismo, se evidenció que la elicitación previa al proceso de inoculación estimuló este proceso en la variedad susceptible, lo que le permitió estar preparada para el desafío contra el patógeno. El conocimiento generado en este trabajo complementa los trabajos desarrollados en este modelo, y evidenció el papel central que tienen los flavonoides, especialmente los glicosilados de tipo flavonol, en la inducción de resistencia usando elicitores de origen biótico
dc.languagespa
dc.publisherBogotá - Ciencias - Maestría en Ciencias - Bioquímica
dc.publisherDepartamento de Química
dc.publisherUniversidad Nacional de Colombia - Sede Bogotá
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dc.rightsAtribución-NoComercial 4.0 Internacional
dc.rightsAcceso abierto
dc.rightshttp://creativecommons.org/licenses/by-nc/4.0/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightsDerechos reservados - Universidad Nacional de Colombia
dc.titleEfecto de la aplicación de elicitores de origen biótico en la biosíntesis de flavonoides en clavel (Dianthus caryophyllus L) durante la interacción con fusarium oxysporum f sp. dianthi
dc.typeOtro


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