Efecto de la cepa biocontroladora IBUN 2755 sobre la población de Burkholderia glumae en plantas de arroz (Oryza sativa L.)

dc.contributorUribe Vélez, Daniel
dc.contributorMicrobiología Agrícola
dc.creatorPerea Molina, Paula Andrea
dc.date.accessioned2020-08-15T02:25:39Z
dc.date.available2020-08-15T02:25:39Z
dc.date.created2020-08-15T02:25:39Z
dc.date.issued2019-07-28
dc.identifierhttps://repositorio.unal.edu.co/handle/unal/78052
dc.description.abstractLa cepa de Bacillus velezensis IBUN 2755 fue aislada de la rizósfera de papa criolla, la cual posee capacidad de control biológico contra Burkholderia glumae, un patógeno muy limitante en el cultivo de arroz. Semillas de arroz infectadas con B. glumae y tratadas con la cepa IBUN 2755, reducen la severidad de los síntomas generados a niveles similares a los del control sin inocular. Por otra parte, en plantas infectadas con el patógeno la presencia de B. glumae se mantiene constante durante los estadios fenológicos analizados, sin embargo, en plantas tratadas con IBUN 2755 su población se ve reducida e incluso indetectable en período de floración. Si bien estos atributos sugieren control biológico contra B. glumae, actualmente no es claro cuáles son los mecanismos llevados a cabo por la cepa IBUN 2755 para lograr el control de este patógeno. Adicionalmente, las plantas inoculadas presentan atributos de promoción de crecimiento vegetal mostrando una longitud mayor a pesar de estar en presencia del patógeno. Tanto IBUN 2755 como B. glumae tienen quimiotaxis frente a los compuestos presentes en los exudados radiculares de arroz secretados a los 7, 14, 21 y 28 días, en la variedad Fedearroz 2000, aunque IBUN 2755 tiene una mayor quimiotaxis por los exudados secretados al día 7. Por otra parte, IBUN 2755 presenta una marcada quimiotaxis por alanina y serina, baja por prolina e indetectable por leucina, en contraste con B. glumae que presenta quimiotaxis por todos los compuestos presentes en los exudados de arroz probados, sin presentar diferencias estadísticas entre estos. Ambas poblaciones mantienen un patrón similar de atracción hacia estos exudados sugiriéndose un posible solapamiento de nicho. IBUN 2755 es capaz de colonizar como endófito plantas de arroz hasta el día 28 tanto en raíz como vástago bajo un sistema controlado estéril, así mismo puede mantener su población superficialmente en la planta. Por otra parte, la cepa forma biofilm en caldo LB y LB-MG a las 24 horas y se mantiene firme hasta las 48 horas, sin embargo, B. glumae no es capaz de formar biofilm en las condiciones analizadas, sugiriendo que el efecto de la cepa IBUN 2755 en la reducción de las poblaciones de B. glumae en plantas de arroz y su estrategia de control está asociada a una mayor capacidad de colonización mediada por una mayor capacidad de formación de biopelícula y competencia por espacio que B. glumae. Palabras clave: (Quimiotaxis, Biofilm, B. velezensis, B. glumae, Arroz)
dc.description.abstractThe strain Bacillus velezensis IBUN 2755 was isolated from the rhizosphere of Criolla potato. This strain has the ability of biological control against Burkholderia glumae a limiting pathogen in rice crops. Rice seeds infected with B. glumae and treated with IBUN 2755 reduce the severity of the symptoms generated by the disease, showing the same characteristics when is compared with healthy plants. On the other hand, the presence of B. glumae remains constant during the phenological stages analyzed, however, in plants treated with IBUN 2755 its population is reduced and even undetectable during flowering. Even if those features suggest biological control against B. glumae, nowadays is not clear what are the mechanisms used by the strain IBUN 2755 to achieve the pathogen control. Moreover, the plants inoculated with IBUN 2755 showed plant growth promotion features, showing a longer length despite being in pathogen presence. Both IBUN 2755 and B. glumae have chemotaxis against compounds present in rice exudates and against rice root exudates secreted at 7, 14, 21 and 28 days, rice variety Fedearroz 2000, though IBUN 2755 has higher chemotaxis for exudates secreted at day 7. On the other hand, IBUN 2755 presents a chemotaxis towards alanine and serine, low by proline and undetectable by leucine, in contrast to B. glumae that shows chemotaxis for all the compounds present in the tested rice exudates, without presenting statistical differences between them. Both microorganism have a similar attraction patron towards exudates suggesting a possible niche overlapping. IBUN 2755 is able to colonize rice plants as an endophyte until day 28 in both root and stem under a sterile controlled system, and it can also keep its population superficially in the plant. On the other hand, the strain forms biofilm in LB and LB-MG broth 24 hours and remains firm until 48 hours, however, B. glumae is not able to form biofilm under the analysed conditions, suggesting that the effect of the strain IBUN 2755 in the reduction B. glumae population in rice plants and its control strategy are associate to a better colonization ability mediated by a biofilm formation ability and space competition than B. glumae. Keywords: (Chemotaxis, Biofilm, B. velezensis, B. glumae)
dc.languagespa
dc.publisherBogotá - Ciencias - Maestría en Ciencias - Microbiología
dc.publisherUniversidad Nacional de Colombia - Sede Bogotá
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dc.titleEfecto de la cepa biocontroladora IBUN 2755 sobre la población de Burkholderia glumae en plantas de arroz (Oryza sativa L.)
dc.typeOtro


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