CELSR en los procesos de polaridad en una línea celular de cáncer de seno

dc.contributorUmaña Pérez, Yadi Adriana
dc.contributorGrupo de Investigación en Hormonas
dc.creatorCorrea Sánchez, Andrés Felipe
dc.date.accessioned2021-01-26T15:07:13Z
dc.date.available2021-01-26T15:07:13Z
dc.date.created2021-01-26T15:07:13Z
dc.date.issued2020-08-18
dc.identifierhttps://repositorio.unal.edu.co/handle/unal/78921
dc.description.abstractCadherins are a superfamily of adhesion molecules with functions in cell recognition, tissue morphogenesis and tumour suppression. Among the members of this family, there is a subgroup of adhesion receptors coupled to G protein, that contain cadherin repeats on their N-terminus, called EGF LAG seven-pass G- typed receptor or CELSR. Three types of non-classical cadherins exist (CELSR 1,2 and 3), and they are involved in the mechanism of cell polarity by activation of the non-canonical Wnt signalling pathway in the epithelial tissue. Recently, these cadherins have been found in the hematopoietic tissue, but their functional role is not clear. Due to the importance of adhesion molecules to promote migration processes in cancer, we studied the presence of CELSR family in an epithelial cell line derived from breast cancer MCF-7 and in cell lines from acute lymphoid leukaemia Jurkat and CCRF-CEM to evaluate functional involvement. A high expression level of the CELSR receptor was found in the MCF-7 cell line in comparison to the epithelial cell lines. Triple silencing of CELSR 1, 2 and 3 in MCF-7 decreased cell migration in response to the activation to the non-canonical Wnt signalling pathway, decreased of production of diacylglycerol was also seen without altering proliferation and cell viability.
dc.description.abstractLas cadherinas son una superfamilia de moléculas de adhesión con funciones en reconocimiento celular, morfogénesis tisular y supresión tumoral. A esta familia pertenece un subgrupo de receptores de adhesión acoplados a proteína G, que contienen repeticiones de cadherina en su N-terminal, las cadherinas EGF LAG seven pass G-type receptor o CELSR. De estas cadherinas no clásicas existen 3 miembros (CELSR 1 a 3), y están involucradas en mecanismos de polaridad celular por activación de la vía de señalización Wnt no canónica en el tejido epitelial. Recientemente, estas cadherinas se encontraron en el tejido hematopoyético, aunque su implicación funcional aún no es clara. Debido a la importancia que tienen las moléculas de adhesión en la promoción de los procesos migratorios en cáncer, este trabajo se centró en identificar la presencia de los miembros de la familia CELSR en la línea epitelial derivada de cáncer de seno MCF-7 y en las líneas celulares de leucemia linfoide aguda Jurkat y CCRF-CEM y evaluar su implicación funcional. Se encontró un mayor nivel de expresión de las cadherinas en la línea celular MCF-7 en comparación con líneas de leucemia. El silenciamiento triple de CELSR 1, 2 y 3, en MCF-7, disminuyó la migración celular en respuesta a la activación de la vía de señalización Wnt no canónica disminuyendo a su vez, la producción de diacilglicerol sin alterar la viabilidad ni la proliferación celular
dc.languagespa
dc.publisherBogotá - Ciencias - Maestría en Ciencias - Bioquímica
dc.publisherDepartamento de Química
dc.publisherUniversidad Nacional de Colombia - Sede Bogotá
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dc.rightsAcceso abierto
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dc.titleCELSR en los procesos de polaridad en una línea celular de cáncer de seno
dc.typeOtro


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