Efecto de la inoculación de bacterias promotoras de crecimiento vegetal en pasto Ryegrass perenne sometido a déficit hídrico

dc.contributorEstrada Bonilla, Germán Andrés
dc.contributorAlvarez Flórez, Fagua
dc.contributorSistemas Agropecuarios Sostenibles
dc.creatorCortés Patiño, Sandra Lucía
dc.date.accessioned2020-08-05T15:51:04Z
dc.date.available2020-08-05T15:51:04Z
dc.date.created2020-08-05T15:51:04Z
dc.date.issued2020-01-18
dc.identifierhttps://repositorio.unal.edu.co/handle/unal/77940
dc.description.abstractPerennial ryegrass is a forage commonly used in template regions of Colombia for livestock feeding. It has high nutritional values but needs large amounts of water to achieve an optimal growth. The aim of this work was to determine if the inoculation of plant growth-promoting bacteria (PGPB), individually or in dual inoculations, can help to improve the response of this grass to water deficit. First, an assay was performed to select the PGPB that could influence biomass production under 10 days of water deficit. Three co-inoculations were selected: 1) Herbaspirillum sp. AP02 and Herbaspirillum sp. AP21; 2) Herbaspirillum sp AP02. and Pseudomonas sp. N7; and 3) Herbaspirillum sp. AP21 and Azospirillum sp. D7. In vitro assays suggested that these bacteria can colonize ryegrass internal tissues and improve seedlings growth under simulated water deficit (p<0.05). Plant growth-promoting activities evaluation showed an increase in indolic compounds for some dual inoculations, and a negative effect in exopolysaccharide production (p<0.05). Finally, a greenhouse experiment was performed to test the ability of these bacteria to modify some physiological, biochemical and morphological traits of this grass under water deficit. The bacteria had an effect in shoot biomass production, stomatal conductance, proline accumulation and chlorophyll pigments during the days of water deficit (p<0.05). They also improved leaves water status after rehydration, about 12% higher than the control. These results might be the first step to find a PGPB consortium that can help improve the response of ryegrass pastures to water deficit.
dc.description.abstractEl ryegrass perenne es un pasto comúnmente utilizado para alimentación ganadera en el altiplano cundiboyacense. Cuenta con buenas cualidades nutricionales, pero requiere altas cantidades de agua para un óptimo desarrollo. El objetivo de este trabajo fue evaluar si el uso de bacterias promotoras de crecimiento vegetal (PGPB), en forma individual o en coinoculaciones duales, mejora la respuesta de este pasto al déficit hídrico. Se realizó un ensayo inicial con 17 tratamientos y, luego de 10 días sin agua, se seleccionaron tres coinoculaciones debido a su capacidad promotora de crecimiento vegetal (p<0.05): 1) Herbaspirillum sp. AP02 y Herbaspirillum sp. AP21, 2) Herbaspirillum sp. AP02 y Pseudomonas sp. N7, y 3) Herbaspirillum sp. AP21 y Azospirillum sp. D7. Ensayos in vitro con estas bacterias sugieren su capacidad de colonización de tejidos vegetales y de mejorar el desarrollo de plántulas bajo déficit hídrico (p<0.05). En algunas coinoculaciones aumentó la producción de compuestos indólicos in vitro y disminuyó la producción de exopolisacáridos (p<0.05). Finalmente, se realizó una evaluación en invernadero de la capacidad de estas coinoculaciones de modificar algunas respuestas fisiológicas, bioquímicas y morfológicas de este pasto al déficit hídrico. Las bacterias mostraron un efecto en la biomasa, conductancia estomática, acumulación de prolina y pigmentos fotosintéticos durante los días de déficit hídrico (p<0.05). Además, mejoraron el estado hídrico foliar luego de la rehidratación, 12% más que el testigo. Estos resultados sugieren que el uso de coinoculaciones de PGPB como una potencial estrategia para mejorar la resistencia del ryegrass a condiciones de déficit hídrico.
dc.languagespa
dc.publisherBogotá - Ciencias - Maestría en Ciencias - Biología
dc.publisherUniversidad Nacional de Colombia - Sede Bogotá
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dc.rightsAtribución-NoComercial 4.0 Internacional
dc.rightsAcceso abierto
dc.rightshttp://creativecommons.org/licenses/by-nc/4.0/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightsDerechos reservados - Universidad Nacional de Colombia
dc.titleEfecto de la inoculación de bacterias promotoras de crecimiento vegetal en pasto Ryegrass perenne sometido a déficit hídrico
dc.typeDocumento de trabajo


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