dc.creator | Garrido Palma, Maritza | |
dc.creator | Torres, Ignacio | |
dc.creator | Ávila, Alba | |
dc.creator | Chnaiderman Figueroa, Jonas | |
dc.creator | Valenzuela Valderrama, Manuel | |
dc.creator | Aramburo, José | |
dc.creator | Oróstica, Lorena | |
dc.creator | Durán Jara, Eduardo | |
dc.creator | Lobos González, Lorena | |
dc.creator | Romero Osses, Carmen | |
dc.date.accessioned | 2021-04-09T15:58:04Z | |
dc.date.available | 2021-04-09T15:58:04Z | |
dc.date.created | 2021-04-09T15:58:04Z | |
dc.date.issued | 2020 | |
dc.identifier | Int. J. Mol. Sci. 2020, 21, 7657 | |
dc.identifier | 10.3390/ijms21207657 | |
dc.identifier | https://repositorio.uchile.cl/handle/2250/179035 | |
dc.description.abstract | Nerve Growth Factor (NGF) and its high-affinity receptor tropomyosin receptor kinase A (TRKA) increase their expression during the progression of epithelial ovarian cancer (EOC), promoting cell proliferation and angiogenesis through several oncogenic proteins, such as c-MYC and vascular endothelial growth factor (VEGF). The expression of these proteins is controlled by microRNAs (miRs), such as miR-145, whose dysregulation has been related to cancer. The aims of this work were to evaluate in EOC cells whether NGF/TRKA decreases miR-145 levels, and the effect of miR-145 upregulation. The levels of miR-145-5p were assessed by qPCR in ovarian biopsies and ovarian cell lines (human ovarian surface epithelial cells (HOSE), A2780 and SKOV3) stimulated with NGF. Overexpression of miR-145 in ovarian cells was used to evaluate cell proliferation, migration, invasion, c-MYC and VEGF protein levels, as well as tumor formation and metastasis in vivo. In EOC samples, miR-145-5p levels were lower than in epithelial ovarian tumors. Overexpression of miR-145 decreased cell proliferation, migration and invasion of EOC cells, changes that were concomitant with the decrease in c-MYC and VEGF protein levels. We observed decreased tumor formation and suppressed metastasis behavior in mice injected with EOC cells that overexpressed miR-145. As expected, ovarian cell lines stimulated with NGF diminished miR-145-5p transcription and abundance. These results suggest that the tumoral effects of NGF/TRKA depend on the regulation of miR-145-5p levels in EOC cells, and that its upregulation could be used as a possible therapeutic strategy for EOC. | |
dc.language | en | |
dc.publisher | MDPI | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
dc.source | International Journal of Molecular Sciences | |
dc.subject | MicroRNA-145 | |
dc.subject | NGF | |
dc.subject | TRKA | |
dc.subject | Epithelial ovarian cancer | |
dc.subject | C-MYC | |
dc.subject | VEGF | |
dc.title | NGF/TRKA Decrease miR-145-5p Levels in Epithelial Ovarian Cancer Cells | |
dc.type | Artículo de revista | |