Artículos de revistas
UHPLC-MS chemical fingerprinting and antioxidant, antiproliferative, and enzyme inhibition potential of gaultheria pumila berries
Fecha
2021Registro en:
Metabolites 2021, 11, 523
10.3390/metabo11080523
Autor
Fernández Galleguillos, Carlos
Quesada Romero, Luisa
Puerta, Adrián
Padrón, José M.
Souza, Ernane
Romero Parra, Javier Hernán
Simirgiotis, Mario J.
Institución
Resumen
Gaultheria pumila (Ericaceae) (known as Chaura or Mutilla) is a Chilean native small
shrub that produces berry fruits consumed by local Mapuche people. In this study, the chemical
fingerprinting and antioxidant, enzyme inhibition, and antiproliferative activities of the berries
were investigated for the first time. Thirty-six metabolites were identified in the fruits by ultra-high
performance liquid chromatography-photodiode array detection, hyphenated with Orbitrap mass
spectrometry analysis (UHPLC-DAD-Orbitrap-MS). Metabolites, included anthocyanins, phenolic acids, flavonoids, iridoids, diterpenes, and fatty acids. Moderate inhibitory activities against
acetylcholinesterase (7.7 ± 0.3 µg/mL), butyrylcholinesterase (34.5 ± 0.5 µg/mL), and tyrosinase
(3.3 ± 0.2 µg/mL) enzymes were found. Moreover, selected major compounds were subjected to
docking assays in light of their experimental inhibition. Results indicated that hydrogen bonding,
π–π interaction, and a salt bridge interaction contributed significantly. Gaultheria pumila berries
showed a total phenolic content of 189.2 ± 0.2 mg of gallic acid equivalents/g, total flavonoid content
of 51.8 ± 0.1 mg quercetin equivalents/g, and total anthocyanin content of 47.3 ± 0.2 mg of cianydin3-glucoside equivalents/g. Antioxidant activity was assessed using DPPH (92.8 ± 0.1 µg/mL), FRAP
(134.1 ± 0.1 µmol Trolox equivalents/g), and ORAC (4251.6 ± 16.9 µmol Trolox equivalents/g)
assays. Conversely, Gaultheria pumila showed a scarce antiproliferative potential against several
solid human cancer cells. Our findings suggest that Gaultheria pumila berries have several bioactive
metabolites with inhibitory effects against acetylcholinesterase, butyrylcholinesterase, and tyrosinase,
and have the potential for use in food supplements.