Artículo de revista
Fatty acid and lipid metabolism in liver of pregnant mice and their offspring is influenced by unbalanced folates/vitamin B12 diets
Fecha
2020Registro en:
Prostaglandins, Leukotrienes and Essential Fatty Acids 154 (2020) 102057
10.1016/j.plefa.2020.102057
Autor
Castaño Moreno, Erika
Castillo, Valeska
Peñailillo, Reyna
Llanos Silva, Miguel
Valenzuela Báez, Rodrigo
Ronco Macchiavello, Ana
Institución
Resumen
Micronutrients (folates and vitamin B12) and long chain polyunsaturated fatty acids (LC-PUFAs) are linked through the one carbon cycle. We studied the effects of pre and postnatal high FA/low B12 diets (HFLB12) on hepatic fatty acid metabolism. Pregnant C57BL/6 mice were divided in two groups: control (2 mg folic acid: FA/25 mu g vitamin B12/Kg food) and HFLB12 diets (8 mg FA/5 mu g vitamin B12/Kg food). Offspring continued on the same diets until 60 days old. We determined hepatic fatty acid profile in dams and offspring and the expression of PPARa, Cpt-1, Acox-1 and Fas and the enzymatic activity of desaturases, all involved in lipid metabolism. In liver of dams, the HFHB12 diet decreased total fatty acids and desaturase activities; in offspring, effects were opposite, being more noticeable in females. Prenatal and postnatal unbalanced folic acid/B12 diets play a crucial role in regulating genes and enzymes involved in lipid metabolism in liver of dams and their offspring in adulthood.