dc.creator | Rojas Benítez, Diego | |
dc.creator | Allende Connelly, Miguel L. | |
dc.date.accessioned | 2020-05-20T20:54:27Z | |
dc.date.available | 2020-05-20T20:54:27Z | |
dc.date.created | 2020-05-20T20:54:27Z | |
dc.date.issued | 2020 | |
dc.identifier | Int. J. Mol. Sci. (2020); 21(3): 925 | |
dc.identifier | 10.3390/ijms21030925 | |
dc.identifier | https://repositorio.uchile.cl/handle/2250/174872 | |
dc.description.abstract | Transfer RNAs (tRNAs) are the most post-transcriptionally modified RNA species. Some of these modifications, especially the ones located in the anti-codon loop, are required for decoding capabilities of tRNAs. Such is the case for 5-methoxy-carbonyl-methyl-2-thio-uridine (mcm(5)s(2)U), synthetized by the Elongator complex. Mutants for its sub-units display pleiotropic phenotypes. In this paper, we analyze the role of elp3 (Elongator catalytic sub-unit) in zebrafish development. We found that it is required for trunk development; elp3 knock-down animals presented diminished levels of mcm(5)s(2)U and sonic hedgehog (Shh) signaling activity. Activation of this pathway was sufficient to revert the phenotype caused by elp3 knockdown, indicating a functional relationship between Elongator and Shh through a yet unknown molecular mechanism. | |
dc.language | en | |
dc.publisher | MDPI | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
dc.source | International Journal of Molecular Sciences | |
dc.subject | Elongator complex | |
dc.subject | tRNA modification | |
dc.subject | Zebrafish | |
dc.title | Elongator subunit 3 (Elp3) Is required for Zebrafish trunk development | |
dc.type | Artículo de revista | |