First Molecular Characterization of Trichostrongylus colubriformis Infection in Rural Patients from Chile

dc.creatorHidalgo, Alejandro
dc.creatorGacitua, Pablo
dc.creatorMelo, Angelica
dc.creatorOberg, Carlos
dc.creatorHerrera, Christian
dc.creatorFonseca Salamanca, Flery
dc.date2020
dc.date2021-04-30T16:58:24Z
dc.date2021-04-30T16:58:24Z
dc.date.accessioned2021-06-14T22:06:15Z
dc.date.available2021-06-14T22:06:15Z
dc.identifierACTA PARASITOLOGICA,Vol.65,790-795,2020
dc.identifierhttp://repositoriodigital.uct.cl/handle/10925/3665
dc.identifier10.2478/s11686-020-00206-1
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3300582
dc.descriptionPurpose The aim was to characterize the infection by Trichostrongylus spp. in patients from Chile using a combination of molecular detection techniques and phylogenetic analysis relating the findings to clinical and epidemiological reports of the patients Methods Strongylid eggs were detected in seven patients by coproparasitological techniques. From each sample a fragment of the ITS-2 ribosomal gene was amplified by PCR, sequenced and analyzed by the Neighbor-Joining method. Results All the sequences and phylogenetic clusters corresponded to T. colubriformis. Two samples presented a single nucleotide polymorphism showing two possible haplotypes. Six patients presented gastrointestinal symptoms. All of them lived on farms and used sheep manure as fertilizer. Conclusion T. colubriformis was the strongylid involved in the infections of these Chilean patients associated with the presence of livestock and agricultural practices that favor infection by this type of nematode.
dc.languageen
dc.publisherSPRINGER INTERNATIONAL PUBLISHING AG
dc.sourceACTA PARASITOLOGICA
dc.subjectTrichostrongylus colubriformis
dc.subjectPCR
dc.subjectStrongylid
dc.subjectLivestock
dc.subjectManure
dc.titleFirst Molecular Characterization of Trichostrongylus colubriformis Infection in Rural Patients from Chile
dc.typeArticle


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