| dc.creator | Medina Schwerter, Gustavo | |
| dc.creator | Neves, Patricia | |
| dc.creator | Flores Martin, Sandra | |
| dc.creator | Manosalva, Carolina | |
| dc.creator | Andaur Rademacher, Marcela | |
| dc.creator | Otth, Carola | |
| dc.creator | Lincopan, Nilton | |
| dc.creator | Fernandez, Heriberto | |
| dc.date | 2019 | |
| dc.date | 2021-04-30T16:47:51Z | |
| dc.date | 2021-04-30T16:47:51Z | |
| dc.date.accessioned | 2021-06-14T22:05:30Z | |
| dc.date.available | 2021-06-14T22:05:30Z | |
| dc.identifier | ARCHIVES OF MICROBIOLOGY,Vol.201,1075-1083,2019 | |
| dc.identifier | http://repositoriodigital.uct.cl/handle/10925/3586 | |
| dc.identifier | 10.1007/s00203-019-01678-0 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/3300290 | |
| dc.description | Arcobacter butzleri is an emerging foodborne zoonotic pathogen that has been isolated from environmental water sources. This pathogen establishes in vitro endosymbiotic relationships with Acanthamoeba castellanii, a free-living amoeba found in environmental matrices such as soil and water. The principal aim of this study was to analyse the transcriptional pattern of flagellar (flaA-flaB-flgH-motA) and other putative virulence genes (ciaB-cadF-mviN-pldA) of A. butzleri during its interaction with A. castellanii by quantitative real-time PCR. The transcriptional analysis showed up-regulation of all genes analysed before A. butzleri became established as an endocytobiont of A. castellanii. In contrast, while A. butzleri remains an endocytobiont, a significant and sustained decrease in the transcription of all analysed genes was observed. Our findings suggest that A. butzleri requires a biphasic transcriptional pattern of flagellar and other putative virulence genes to establish an endosymbiotic relationship with A. castellanii. | |
| dc.language | en | |
| dc.publisher | SPRINGER | |
| dc.source | ARCHIVES OF MICROBIOLOGY | |
| dc.subject | Arcobacter | |
| dc.subject | Acanthamoeba | |
| dc.subject | Virulence genes | |
| dc.subject | Flagellar genes | |
| dc.subject | Endosymbiosis | |
| dc.title | Transcriptional analysis of flagellar and putative virulence genes of Arcobacter butzleri as an endocytobiont of Acanthamoeba castellanii | |
| dc.type | Article | |
