Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp

dc.creatorBernabe, Patricia
dc.creatorBecheran, Liliam
dc.creatorCabrera Barjas, Gustavo
dc.creatorNesic, Aleksandra
dc.creatorAlburquenque, Claudio
dc.creatorTapia, Cecilia, V
dc.creatorTaboada Valdés, Edelio
dc.creatorAlderete, Joel
dc.creatorDe Los Ríos Escalante, Patricio
dc.date2020
dc.date2021-04-30T17:06:06Z
dc.date2021-04-30T17:06:06Z
dc.date.accessioned2021-06-14T22:01:05Z
dc.date.available2021-06-14T22:01:05Z
dc.identifierINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,Vol.149,962-975,2020
dc.identifierhttp://repositoriodigital.uct.cl/handle/10925/4017
dc.identifier10.1016/j.ijbiomac.2020.01.126
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3298758
dc.descriptionIn the present work, the chemical composition of the Chilean freshwater crab Aegla cholchol exoskeleton was studied for the first time. alpha-Chitin was isolated from three main body parts (pincers, legs, carapace), and its content ranged from 9.0-10.4% (w/w). Moreover, chitosan was extracted by alkaline treatment at different temperature and time regimes. Aegla cholchol exoskeleton, chitin and chitosans were characterized by FTIR, TGA, and SEM. Chemical structure of chitin and chitosan was confirmed by NMR spectroscopy. Chitosan molecular weight determinations were carried out by GPC. The obtained chitosan samples had a degree of N-acetylation (DA) between 4 and 15% and molecular weight (Mw) in the range of 65-201 kDa. The antifungal activity of the chitosan samples and the chitooligomer were tested toward twenty isolated clinical strains of Candida yeast. Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida Iropicalis, Candida glabrata, and Candida parapsilosis. Results suggest that Aegla cholchol is an excellent natural source for production of bioactive materials with potential applications in the health system, to prevent infections associated with Candida strains. (C) 2020 Elsevier B.V. All rights reserved.
dc.languageen
dc.publisherELSEVIER
dc.sourceINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
dc.subjectAegla cholchol freshwater crab
dc.subjectChitosan
dc.subjectChitin
dc.subjectCharacterization
dc.subjectAntifungal activity
dc.subjectCandida yeast
dc.titleChilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp
dc.typeArticle


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