Antibacterial properties of chitosan-based coatings are affected by spacer-length and molecular weight

dc.creatorVaz, Juliana M.
dc.creatorTaketa, Thiago B.
dc.creatorHernandez Montelongo, Jacobo
dc.creatorChevallier, Pascale
dc.creatorCotta, Monica A.
dc.creatorMantovani, Diego
dc.creatorBeppu, Marisa M.
dc.date2018
dc.date2021-04-30T16:58:24Z
dc.date2021-04-30T16:58:24Z
dc.date.accessioned2021-06-14T22:00:14Z
dc.date.available2021-06-14T22:00:14Z
dc.identifierAPPLIED SURFACE SCIENCE,Vol.445,478-487,2018
dc.identifierhttp://repositoriodigital.uct.cl/handle/10925/3663
dc.identifier10.1016/j.apsusc.2018.03.110
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3298463
dc.descriptionChitosan is a biopolymer with antibacterial properties, which are dependent on its molecular weight (Mw) and its degree of deacetylation (DDA). When grafted on surfaces as a coating, chitosan antibacterial efficiency is also dependent on the polymer chain conformation on the surface, as the amine groups, responsible of the antibacterial effect, should be available for contact with bacteria. To investigate this behavior, chitosans with different Mw were grafted onto plasma aminated surfaces through three different spacers: glutaric anhydride (GA), poly(ethylene-glycol) bis(carboxymethyl) ether (PEGb), and poly (ethylene-alt-maleic anhydride) (PA). The grafting efficiency was evaluated by X-ray Photoelectron Spectroscopy (XPS), contact angle and Rose Bengal test, while morphological features were assessed by profilometry analyses. Results evidenced a clear influence of the anchor arm length and of the Mw of chitosan both on the grafting efficiency and on the antibacterial behavior. PA CHIMW surface exhibited a better antibacterial response compared to GA and PEGb, which could be correlated to a denser coating coverage as seen by XPS and profilometry results. Further, PA CHIMW coating displayed a higher amine density, thus promoting the interaction with the bacteria cell wall. Based on these results, chitosan-based coatings can then be extended to a wide range of antibacterial applications. (C) 2018 Elsevier B.V. All rights reserved.
dc.languageen
dc.publisherELSEVIER SCIENCE BV
dc.sourceAPPLIED SURFACE SCIENCE
dc.subjectChitosan-based coatings
dc.subjectGrafting
dc.subjectSurface modification
dc.subjectInterface characterization
dc.subjectAntibacterial tests
dc.subjectXylella fastidiosa
dc.titleAntibacterial properties of chitosan-based coatings are affected by spacer-length and molecular weight
dc.typeArticle


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