| dc.contributor | Mohamed Salem, Abdelfattah Zeidan | |
| dc.contributor | Estrada Zúñiga, María Elena | |
| dc.creator | Gutiérrez Morales, Andrea | |
| dc.date | 2018-11-13T01:40:30Z | |
| dc.date | 2018-11-13T01:40:30Z | |
| dc.date | 2018-04 | |
| dc.date.accessioned | 2019-05-28T21:56:45Z | |
| dc.date.available | 2019-05-28T21:56:45Z | |
| dc.identifier | http://hdl.handle.net/20.500.11799/95125 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/2908267 | |
| dc.description | The desideratum aim of the present context was to assess the biopotency of methanolic extracts of Eichhornia
crassipes (E. crassipes), Pistacia vera (P. vera), and Ziziphus amole (Z. amole) leaves against various staphylococcal
strains, and to quantify the phenolics as well as saponin content in them. The antibacterial activity of various
concentrations (62.5–1000 μg/mL) of plant extracts was tested against control clinical strains (Staphylococcus
aureus ATCC 25923, S. aureus ATCC 29213, and S. aureus ATCC 43300), methicillin-resistant S. aureus (MRSA1
and MRSA2), oxacillin sensitive S. aureus (SOSA1 and SOSA2), and coagulase-negative Staphylococcus epidermidis
(CoNS1, CoNS2, and CoNS3) using disc diffusion assay. Leaf extracts of the three plants exhibited pronounced
growth inhibitory characteristics against staphylococci in a dose dependent manner. E. crassipes extract depicted
the highest relative percentage inhibition values against control clinical strains (68.6 ± 0.5%), while P. vera
(68.6 ± 0.3%) and Z. amole (74.79 ± 0.3%) extracts showed pronounced relative inhibition values against
staphylococcal strains isolated from cattle. Total phenols and saponin content of leaf extracts were investigated
by standard in vitro methods. The methanolic extracts of these plants were found to comprise substantial content
of phenolics and saponin at varying levels. The highest value of phenolics was estimated in P. vera extract
(60.0 ± 1.3 mg gallic acid/g extract), followed by Z. amole (33.6 ± 1.4 mg gallic acid/g extract), and E.
crassipes (23.0 ± 1.3 mg gallic acid/g extract). Saponin content for P. vera, Z. amole, and E. crassipes extracts
were estimated as 41.0 ± 1.3, 35.8 ± 1.3, and 25.0 ± 1.2 mg diosgenin/g extract, respectively. The outcome
of this study suggested the exploitation of methanolic extract of P. vera, Z. amole, and E. crassipes leaves for their
possible application in ethnomedicine, particularly as drugs preparation against staphylococcal infections. In
conclusion, the study indicates the biopotency of these plants against pathogenic MRSA present in cattle, and
SOSA as well as CoNS bacteria present in rabbits, which could be a serious issue for livestock. | |
| dc.language | eng | |
| dc.publisher | Universidad Autonoma del Estado de México | |
| dc.rights | restrictedAccess | |
| dc.rights | https://creativecommons.org/licenses/by-nc/4.0/ | |
| dc.rights | restrictedAccess | |
| dc.rights | https://creativecommons.org/licenses/by-nc/4.0/ | |
| dc.subject | Research Subject Categories::VETERINARY MEDICINE | |
| dc.title | Anti-staphylococcal properties of Eichhornia crassipes, Pistacia vera, and Ziziphus amole leaf extracts: isolates from cattle and rabbits | |
| dc.type | Tesis | |
