ESTERILIZACIÓN DE HUEVOS Y EFECTO DE LARVAS Lucilia sp (DIPTERA:CALLIPHORIDAE) EN BIOFILMS DE Pseudomona aeruginosa.

Abstract

Chronic wounds are commonly associated with the formation of biofilms, formed of antibiotic-resistant bacteria such as Pseudomonas aeruginosa, for that reason is the importance to find new ways to control such infections, one way is the use of sterile larvae of Lucilia sericata fly (Diptera:Calliphoridae) where the three larval mechanisms of action are: secrete digestive enzymes that selectively dissolve necrotic tissue, disinfect the wound, and thus stimulate wound healing. The larvae used in this treatment should be sterilized in the process are applied in their egg and larval instars first to fight antibiotic-resistant bacteria such as Pseudomonas aeruginosa, therefore the objectives of this study were to evaluate ten methods of sterilization in the eggs of Lucilia cuprina and evaluation of the effect of homogenization and secretions, excretions of Lucilia sericata larvae in biofilms of Pseudomonas aeruginosa PA14. The breeding of flies was taken on the premises of Entomology Research Laboratory of Forensic Science (FLIES Texas A & M University). The results obtained show that: Cochliomyia macellaria showed a high rate of hatching of (+ / -91%) at all times, however Lucilia sericata showed a low rate of emergence in all treatments of a (+ / -48%) . As for the ten treatments, treatment with higher hatching rate was with NaClO with a (86.85%), followed by a 70% ETOH (74.36%) of hatching, the hatching rates were lowest NaClO / Formaldehyde ( 50.08%) and formaldehyde (59.84%). As for the higher rates of infertility, 10% had Lysol (99.70%), followed by NaClO / Formaldehyde with (99.50%) and NaClO with a (97%), the lower the rate of infertility presented Formalin 5 % with (71%). Therefore the NaClO can be used in the sterilization process to ensure a high rate of hatching. As the effect of larvae in biofilms of Pseudomonas aeruginosa, homogenization were performed 50 and 100 larvae, and the same solutions of SE 50 and 100 larvae were concentrated for 24 hours in Pseudomonas aeruginosa PA14 biofilms. The homogenate of the larvae fed sterile sterile liver did not reduce the biofilms of Pseudomonas aeruginosa PA14, as the sterile decreased between 21 and 48%. The SE of sterile and non sterile larvae decreased from 66 to 75%, which makes them a good alternative in reducing biofilms of Pseudomonas aeruginosa.