Thesis
Direccionamiento del metabolismo primario a metabolismo secundario para la evaluación de la producción de actinorrodina por medio de la modulación de citrato sintasa y aconitasa en Streptomyces coelicolor
Autor
Pérez Curiel, Julieta
Institución
Resumen
During the life cycle of Streptomyces can distinguish two types of metabolism, primary and secondary. The primary metabolism is defined as the sum of anabolic and catabolic reactions that result in the maintenance of vital cell processes (synthesis of fatty acids, proteins, lipids, polysaccharides, coenzymes, etc ...) and the increase in total biomass. These processes are essentially identical in all living cells. By contrast, in secondary metabolism products are formed that are not essential for the growth of the organism producing complex chemical structures of the body, usually synthesized at the beginning of the stationary phase. However, products originating usually confer ecological advantages and consequently greater biological competitiveness. The objective of this research was to increase the production of actinorrodiana, secondary metabolite of S. coelicolor, by overexpression and silencing of the two first enzymes of the Krebs cycle, citrate synthase and aconitase. It has been reported overexpression of the enzyme acetyl-CoA carboxylase improving the performance of actinorhodin production, this project results in overexpression of citrate synthase and aconitase actinorhodin production was affected by pH declines presented in the first 48 hrs. For the muting technique using antisense RNA in S. coelicolor so far not been reported using this technique. The study of citrate synthase and aconitase in S. coelicor has been reported by the disruption of genes producing actinorhodin non-producing clones and lethal phenotypes. We present an alternative to generate clones with silenced lethal phenotypes actinorhodin production is influenced by pH values below pH 6 where actinorrodina does not support these values achieved.