| dc.description.abstract | The Mexican agriculture constitutes the main source of food and substantially
serve to provides great export. However, there are various pests and diseases that
affect production, among them the viruses are important by their impact on fruit
yield and quality. The genus begomovirus infect dicotyledonous plants, are
transmitted by whiteflies and the virus type member is Bean golden mosaic virus
(BGMV). Most begomoviruses genus have bipartite genome but some of the has
monopartite.. Weeds play an important role as a source of primary inoculum in the
spread and dissemination of viruses affecting the cultivated plants. Many species
have been implicated as hosts of begomovirus in several countries. Gender sida is
a group of weeds that are distributed in both the old and the new world. In Sinaloa
recently identified and sequenced a new begomovirus infecting Sida rhombifolia
called Sinaloa Mosaic Virus Sida, SiMSV. Recently, most originating in the Old
World begomoviruses have been shown to be associated with a class of satellites
known as betasatellites ssDNA which are half the size of its helper begomovirus
(1.4 kb) and are required for virus help produce the typical symptoms of the
disease in their original hosts. In adittion betasatellites, many diseases are
associated with begomovirus third single-stranded DNA which was called
alphasatellite. However, alphasatellites are essential for virus infection and
appears not to play role in the etiology of diseases with which they are associated.
It is above aims of this study the biological characterization of Sinaloa Mosaic Virus
Sida: seed transmission, host range and association with subviral particles. To
meet this goal, the process used molecular techniques such as PCR, cloning
RFLP. The result was the determination of the ability to spread through seed
Sinaloa mosaic virus sida, which also detected the presence of this virus in
peppers and tomatos crops in the northern state of Sinaloa, was determined
experimentally the ability to infect SiMSV peppers and tomatos by grafting, this is
corroborated by the identification by RFLP and was not detected the presence of
subviral particles alpha and betasatellites its natural host.associated with SiMSV | |
