| dc.description.abstract | The culture of Litopenaeus vannamei is a very important productive activity in
northwestern Mexico. In this research we evaluated the prebiotic inulin and the green
algae Ulva lactuca in the growth, survival and immune system of L. vannamei,
cultured in the laboratory. In the bioassey were used plastic tanks with 80 L of sea
water, constant aeration and 10 shrimp/tank. Inulin and macroalgae were added to the
feed (Camaronina®, 35% protein). Feeding was done ad libitum twice a day, daily
cleaning was done and determination of physicochemical parameters every 3 days.
Water quality and weight determination were carried out at the beginning and end of
the bioassay. We determined the specific growth rate. The three bioassays were
performed in triplicate. Bioassay 1 (62 days, 1.1 ± 0.08 g): I) control diet
(Camaronina); II) Camaronina + 1.25 g inulin/kg feed; III) Camaronina + 2.5 g inulin/kg
feed, IV) Camaronina + 5 g inulin/kg feed; V) Camaronina + 10 g inulin/kg feed. At the
end of the bioassay a PCR was performed to detect WSSV and intestine LAB and
yeast were counted. Bioassay 2 (46 days, 0.9 ± 0.05 g): 1) control, Camaronina +
DO; 2) Camaronina + Ulva (50 g/kg feed), 3) Camaronina + Ulva (100 g/kg feed), 4)
Camaronina + Ulva (150 g/kg feed), and 5) Camaronina + Ulva (200 g/kg feed).
Bioassay 3 (73 days, 1.09 ± 0.07 g): I) control (Camaronina); II) 2.5 g inulin + 20%
Ulva/kg feed; III) 2.5 g inulin/kg feed (bioassay 1); IV) 20% Ulva/kg feed (bioassay 2).
Total hemocyte count and the activity of phenoloxidase (PO) in plasma and
prophenoloxidasa (proPO) in the hemocyte lysate supernatant (HLS) were
determined. The results were statistically analyzed. Inulin did not increase the number
of LAB and yeast in the intestine. Inulin and macroalgae did not affect the growth of
shrimp, but the inulin decreased the prevalence of WSSV. The effect of macroalgae
against WSSV was not determined. The combination of inulin and U. lactuca
significantly increased the number of hemocytes but did not increase phenoloxidase
activity in shrimp. Inulin significantly increased the activity of phenoloxidase. | |
