Article
Self assembled monolayer, as optical transducers, using spiropyran photochromic material
Autor
Rojas López, Marlon
Institución
Resumen
The self assembled monolayers (SAM) have become in the most popular strategy for design and generate surfaces
characterizing by specific functional organic groups. The aimed of this work is applied this SAM as optical transducer in
biosensors. The techniques, Infrared (in ATR mode) and UV/Vis spectroscopy have been used to study the films
generated in each step in the self assembled process. The SAM was generated as follow; first silane group was added to
the glass substrate. After that, the substrates were immersed in a solution containing carbomiide group (EDC). Finally
the spiropyran 1′,3′-Dihydro-8-methoxy-1′,3′,3′-trimethyl-6-nitrospiro[2H-1-benzopyran-2,2′-(2H)-indole] was attached
to functionalized slides. In each process absorbance was analyzed by UV/Vis (270 to 500 nm) and FTIR (650 to 1800
cm-1). In UV, the spectra shows an absorbance band centered at 280 associated to EDC film and a lower intensity band
centered at 380 nm associated to spiropyran. In FTIR spectra, the Si-Si and Si-O bond are present below the 1250 cm-1.
The EDC film shows very weak bands in the region from 1300 to 1800 cm-1. For the spiropyran film the band associated
to the C-N, N-O, C=C, C-H and aromatic ring have a very well defined peaks. Once the transducer bands were detected,
it was immersed in glucose solution; the infrared spectral show bands are associated to glucose in the transducer.