| dc.description.abstract | Spiropyrans materials are very studied for their application in transduction systems
for biosensors. In this work the protocol of generation of multi and mono layers films and the
efficiency of the transduction system are analyzed. The analyses were based on UV/Vis and
Infrared vibracional spectroscopy in Attenuated Total Reflexion mode (ATR). The films were
deposited in glass substrates, using (3-Aminopropyl) trimethoxysilano like linker. The UV/Vis
spectra show that the deposition protocol allows the generation of a homogenous film from the
first layer. Similar results were observed in ATR. The efficiency of the films, when analyzing
them after light UV irradiation, is greater in the samples with two and three layers of deposit,
since its absorption peaks are most intense in UV as in ATR. The efficiency falls from the
fourth deposit layer. In ATR the efficiency is assumed by the vibration of the NO bond at 1338
cm-1. | |
