BÚSQUEDA DE BIOMARCADORES PROTEÍNICOS EN ZIGOTOS, EMBRIONES Y BLASTOCISTOS

Abstract

With the development of this study was intended to implement strategies to assess the embryonic proteome allowing higher rates of success of assisted reproduction treatments. Finding effective and easily applicable criteria based on the examination for embryo selection is a goal in reproductive biology as it allows good results with fewer fertilized oocytes and therefore fewer embryos analyzed, reducing the use of biological material and reducing processing costs which is beneficial to couples wishing to have children through such procedures. The way we approach this problem was doing a search using high performance liquid chromatography in reverse phase (RP-HPLC) that is a powerful tool that has great sensitivity, resolution, uses very little sample, is automatable and is possible to analyze proteins that can be used as targets for preimplantation diagnosis. Protein samples were extracted from zygotes, embryos and blastocysts, the analysis of the chromatograms were observed peaks with retention times for peptides with a wavelength of 220nm. These peptides were analyzed by mass spectrometry.