| dc.description.abstract | In this work, a new, easy and rapid method of
analyzing phenolic compounds in pollen extract, based on
capillary electrophoresis coupled with electrospray ionization
time-of-flight-mass spectrometry (CE–ESI–TOF–MS),
has been developed. A systematic investigation of separation
parameters has been performed with respect to
resolution, sensitivity, analysis time and peak shape. The
electrophoretic parameters and electrospray conditions must
be optimized to obtain reproducible analyses. Using this
method, several important phenolic compounds such as acetinglucoside,
7-O-methylherbacetin-3-sophoroside, galloylglucose,
quercetin-3-sophoroside, apigenin-6,8-di-C-glycoside,
quercetin-3-rutinoside, genistein-7-O-β-D-glucoside, luteolin-7-
O-glucoside, apigenin-7-O-glucoside and 2′,4′,6′-trihydroxy-3′-
formyldihydrochalcone have been determined directly from
pollen extract. The efficiency, the rapidity, the small amounts
of sample required, and the high resolution of CE coupled with
the sensitivity, the selectivity, the accurate masses and the true
isotopic patterns obtained using TOF-MS point to the potential
of this approach for identifying the phenolic compounds
present in pollen. | |
