Thesis
DETERMINACION DE LOS ALELOS PRINCIPALES DE LOS GRUPOS SANGUINEOS DUFFY Y DIEGO
Autor
QFB. ARREYGUE AVILA, MARCELO
Institución
Resumen
In the field of transfusion medicine, it is important to study and determination of the Duffy blood group and Diego in donors and in patients who undergo transfusions or transplants. In the anthropological field are considered as markers of population or genetic identity. There have been some studies regarding the phenotype of the Duffy and Diego systems in the Mexican population, however, to date have not been characterized genotypes to determine the correlation between the two studies and use them as a tool in clinical studies and populations.
Objective: To determine the major alleles of the Duffy and Diego blood groups in a sample of the resident population of the Mexico City metropolitan area. Methods: DNA samples were obtained from donors at Medica Sur Hospital and INPer. We used real-time PCR and allele specific to characterize the alleles FY * A, FY * B, FY * X, FY * NULL, DI * DI * B AY using the LightCycler ®. Hybridization probes were used LightCycler FastStart DNA Master HybProbe and EZ Type ® Red Cell for the determinations.
Results: We studied 403 individuals for the Duffy blood group, frequency phenotypic found to Fya + b-, Fya + b + Fya-b + and Fya-b-was 36.7, 43.5, 17.1 and 2.7% respectively, while for alleles FY * A, FY * B, FY * 0 and FY * X frequencies were 0.543, 0.419, 0.026 and 0.012, respectively. concordance between genotype and phenotype for the Duffy system, where this was 0959, for phenotypes Fya + b + and Fya-b + was 0.971 in both cases, Fya + b-0912 and the null phenotype (Fya-b-) was 0.546, which was in the group that found the majority of discrepancies. The system fenotipicación Diego and genotyping was performed in 247 subjects, detected phenotypes and Day + b + a-b + Di in 17.8 and 82.2% respectively, did not identify individuals with phenotype Day + b-. The allele frequency for the DI * A was 0.085 and 0.915 DI * B, not found DI * A allele in homozygous form in any donor. As the overall agreement for this system of 0972 and 0909 phenotype for Day + b + and 0990 for Dia-b +.
Conclusions: For the population studied Duffy system is more like whites than blacks by the higher frequency of allele FY * A. The predominant mechanism for Duffy null phenotype in our population, the Philippines (FY * 0 on FY * A allele). The Dia antigen of the Diego system, is found in very few individuals at a marker that is considered dangerous in terms of population and transfusion.