dc.contributor | De Nova-Ocampo, Mónica | |
dc.creator | Tolentino-Ruíz, Rocío | |
dc.creator | Montoya-Varela, Diana | |
dc.creator | García-Espitia, Matilde | |
dc.creator | Salas-Benito, Mariana | |
dc.creator | Gutiérrez-Escolano, Ana Lorena | |
dc.creator | Gómez-García, María del Consuelo | |
dc.creator | Figueroa-Arredondo, Paula | |
dc.creator | Salas-Benito, Juan Santiago | |
dc.creator | De Nova-Ocampo, Mónica | |
dc.date.accessioned | 2012-10-01T21:32:08Z | |
dc.date.available | 2012-10-01T21:32:08Z | |
dc.date.created | 2012-10-01T21:32:08Z | |
dc.date.issued | 2012-10 | |
dc.identifier | Tolentino-Ruiz R, Montoya-Varela D, Salas-Benito M, García-Espitia M, Gutiérrez-Escolano A, Gómez-García C, Figueroa-Arredondo P, Salas-Benito J, De Nova-Ocampo M. Development of a multiplex PCR assay to detect gastroenteric pathogens in the feces of mexican children. Curr Microbiol. 2012. 65:361-368 | |
dc.identifier | http://www.repositoriodigital.ipn.mx/handle/123456789/7144 | |
dc.description.abstract | Abstract Acute gastroenteritis (AGE) is a major cause of
childhood morbidity and mortality worldwide; the etiology
ofAGEincludes viruses, bacteria, and parasites.Amultiplex
PCR assay to simultaneously identify human Astrovirus
(HAstV), Calicivirus (HuCVs), Entamoeba histolytica
(E. histolytica), and enteroinvasive Escherichia coli (EIEC)
in stool samples is described. A total of 103 samples were
individually analyzed by ELISA (enzyme-linked immunosorbent
assays) and RT-PCR/PCR. HAstV and HuCVs were
detected in four out of 103 samples (3.8 %) by RT-PCR, but
ELISAs found only one sample as positive for HuCVs
(2.5 %). E. histolytica was identified in two out of 19 samples
(10.5 %) and EIEC in 13 out of 20 samples (70 %) by PCR,
and all PCR products were sequenced to verify their identities.
Our multiplex PCR results demonstrate the simultaneous
amplification of different pathogens such as HAstV,
EIEC, and E. histolytica in the same reaction, though the
HuCVs signal was weak in every replicate. Regardless, this
multiplex PCR protocol represents a novel tool for the identification of distinct pathogens and may provide support for the diagnosis of AGE in children. | |
dc.language | en | |
dc.publisher | Current Microbiology | |
dc.subject | Diagnostico | |
dc.subject | Biología molecular | |
dc.subject | Virología | |
dc.subject | Parasitología | |
dc.subject | Bacteriología | |
dc.title | Development of a multiplex PCR assay to detect gastroenteric pathogens in the feces of mexican children | |
dc.type | Article | |