dc.contributor | Athayde, Margareth Linde | |
dc.contributor | http://lattes.cnpq.br/7866111734540735 | |
dc.contributor | Hoelzel, Solange Cristina da Silva Martins | |
dc.contributor | http://lattes.cnpq.br/3371198135113385 | |
dc.contributor | Mostardeiro, Marco Aurelio | |
dc.contributor | http://lattes.cnpq.br/6195396264565980 | |
dc.creator | Zanon, Ricardo Basso | |
dc.date.accessioned | 2006-08-14 | |
dc.date.available | 2006-08-14 | |
dc.date.created | 2006-08-14 | |
dc.date.issued | 2006-04-07 | |
dc.identifier | ZANON, Ricardo Basso. SECUNDARY METABOLIC OF VERNONIA TWEEDIEANA BAKER. 2006. 180 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2006. | |
dc.identifier | http://repositorio.ufsm.br/handle/1/5948 | |
dc.description.abstract | The species Vernonia tweedieana Baker is an herbaceous plant widely distributed in the
plains of Paraguai, Argentina and south of Brazil and popularly known as assa-peixe .
This plant is used in traditional medicine as an expectorant medicament. So far, this
plant was not studied on the phytochemical and biological point of view. This work is a
contribution to the phytochemical study of the Asteraceae. The leaves of V. tweedieana
Baker were collected in march of 2004, in Ijuí Rio Grande do Sul, Brazil, and identified
by Dr. Geraldo C. Coelho (UNIJUÍ). The respective voucher specimen was deposited in
the herbarium of the Federal University of Santa Maria (UFSM)-RS (code SMDB 9536).
The leaves (1.900 g) were dried in an air circulating stove at 40 ºC, pulverized in mill
and extracted by maceration with 65% EtOH at room temperature for seven days. The
ethanolic extract was filtered and the ethanol was removed. Finally, the extract was
retake in water and partitioned using organics solvents with increased polarity: CH2Cl2,
AcOEt and n-BuOH. We report the isolation and identification of six constituents of the
CH2Cl2 fraction: the triterpenes a-amyrin, b-amyrin and lupeol and the steroids b-
sitosterol, stigmasterol and spinasterol. The flavanone eriodictyoI was isolated from the
AcOEt fraction. The constituents were identified through spectral data of the infra-red,
GC-MS, 1H-NMR, 13C-NMR and DEPT. No deaths and other signs of toxicity and
adverse effect were observed in the evaluation of acute toxicity with doses up to 5.000
mg/kg, that is the maximum dose for acute oral toxicity test for extract of plants. Also,
through DPPH method, AcOEt and n-BuOH fractions of the plant showed good
antioxidant activity with IC50 22.52 and 17.44 mg/mL, respectively. | |
dc.publisher | Universidade Federal de Santa Maria | |
dc.publisher | BR | |
dc.publisher | Farmacologia | |
dc.publisher | UFSM | |
dc.publisher | Programa de Pós-Graduação em Ciências Farmacêuticas | |
dc.rights | Acesso Aberto | |
dc.subject | Vernonia tweedieana Baker | |
dc.subject | Triterpenos | |
dc.subject | Esteróides | |
dc.subject | A e b-amirina | |
dc.subject | Lupeol | |
dc.subject | B-sitosterol | |
dc.subject | Estigmasterol | |
dc.subject | Espinasterol | |
dc.subject | Eriodictiol | |
dc.subject | Triterpenes | |
dc.subject | Steroids | |
dc.subject | A-amyrin | |
dc.subject | B-amyrin | |
dc.subject | Lupeol | |
dc.subject | B-sitosterol | |
dc.subject | Stigmasterol | |
dc.subject | Spinasterol | |
dc.subject | Eriodictyol | |
dc.subject | Acute toxicity | |
dc.subject | Antioxidant activity | |
dc.title | Metabólitos secundários em Vernonia tweedieana Baker | |
dc.type | Dissertação | |