Dissertação
Efeito da quercetina na atividade de ectoenzimas e da acetilcolinesterase em sinaptossomas do córtex cerebral de ratos expostos ao cádmio
Fecha
2012-02-27Registro en:
ABDALLA, Fátima Husein. Effect of the quercetin on the ectoenzymes and
acetylcholinesterase activity in synaptosomes the cerebral cortex of rats exposed to cadmium. 2012. 82 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2012.
Autor
Abdalla, Fátima Husein
Institución
Resumen
Cadmium (Cd) is one of the most toxic heavy metals by their ability to affect the vital
organs like liver, kidney, brain and other. This metal can trigger a framework of
oxidative stress by increase of reactive oxygen species and also affect cholinergic
and purinergic neurotransmission. Quercetin (Querc), a flavonoid found in various
foods has several functions in the body as a therapeutic antioxidant and
neuroprotective action. The aim of this study was to evaluate the activity of
ectoenzymes and acetylcholinesterase (AChE) activity in synaptosomes of the
cerebral cortex of adult rats exposed to cadmium chloride at a dose of 2.5 mg/kg and
treated with Querc at doses of 5, 25 and 50mg/kg, both solutions were orally
administered in a volume of 1 mL/kg for 45 days. Results showed that the hydrolysis
of ATP, ADP, AMP and ADA activity in the synaptosome cerebral cortex in the
Cd/ethanol group increased compared to the saline/ethanol group (p<0.05). The
treatment with all doses of Querc prevented the increase in the hydrolysis of ATP,
ADP, and AMP as well as the ADA activity (p<0.05). In vitro assay with Querc 25 and
50 μM showed a decrease in the activities of these enzymes except of ADA (p<0.05).
The AChE activity ex vivo was not significantly different in the Cd/ethanol group when
compared to the saline/ethanol group. The treatment with Querc 25 and 50 mg/kg
significantly decreased the AChE activity when compared to the Cd/ethanol group
(p<0.05). In vitro assay showed a decrease in the AChE activity only with 100 and
200 μM of Querc (p<0.05). These results suggest that Querc prevented the
alterations caused by Cd in purinergic and cholinergic system. Thus, the results
reported here suggest that Querc is a promising compound that can be clinically
investigated in order to be used in alternative therapies for the treatment of
neurodegenerative diseases or brain diseases associated with poisoning by heavy
metals.