dc.contributor | Athayde, Margareth Linde | |
dc.contributor | http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707843T7 | |
dc.contributor | Augusti, Paula Rossini | |
dc.contributor | http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4778049T7 | |
dc.contributor | Vaucher, Lauren Rosa Crossetti | |
dc.contributor | http://lattes.cnpq.br/7468913314581754 | |
dc.creator | Feltrin, Andrieli Cassel | |
dc.date.accessioned | 2013-05-21 | |
dc.date.available | 2013-05-21 | |
dc.date.created | 2013-05-21 | |
dc.date.issued | 2010-09-23 | |
dc.identifier | FELTRIN, Andrieli Cassel. COMPARATIVE STUDY OF Glycyrrhiza glabra (LICORICE) AND
Periandra dulcis (LICORICE-OF-THE-EARTH). 2010. 88 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2010. | |
dc.identifier | http://repositorio.ufsm.br/handle/1/5939 | |
dc.description.abstract | The Fabaceae family is comprised of approximately 18 000 species in 650 genera, with wide
geographic distribution. The species Glycyrrhiza glabra (licorice) and Periandra dulcis
(licorice-of-the-earth) belong to the family Fabaceae. Licorice displays a variety of
pharmacological activities, such as antiulcer, antiinflammatory, antioxidant, antimicrobial,
among others. In ethnomedicine Brazilian, P. dulcis is used as a substitute/adulterant G.
glabra. This study aimed to differentiate plants for antioxidant activity, the HPLC
chromatographic profile and the antimicrobial activity. Plants roots were ground and
macerated using as solvent ethanol: water (70:30, v/v). Became fractionation of crude
extracts with organic solvents of increasing polarity (CH2Cl2, EtOAc, n-BuOH). The crude
extract and the fractions were investigated for the content of total polyphenols, the
antioxidant activity by DPPH and the inhibition of lipid peroxidation by TBARS method. The
content of total phenolics ranged from 70.29 ± 2.98 to 85.78 ± 1.08 mg/g for P. dulcis and
137.7 ± 1.37 to 83.26 ± 2.71 mg/g to G. glabra. In the DPPH assay, the dichloromethane
fraction was most effective in capturing free radicals in both plants. The butanol fraction of P.
dulcis showed the greatest inhibition of TBARS, with IC50 of 1.38 ± 1.22 μg/mL and better
chart the decay. In G. glabra, the dichloromethane fraction showed the best IC50 of 1.91 ±
0.73 μg/mL, but the best graphic decay was observed in the crude extract. Using the method
proposed by the Brazilian Pharmacopoeia Fourth Edition, it was possible to differentiate the
plants through the HPLC profiles. The absence of glycyrrhizin acid is evidence of falsification.
However, the method does not allow the identification of the plant adulterant. The
antimicrobial activity of the species was similar, varying only for Saccharomyces cerevisiae
and Fusarium proliferatum. The sub-fractionation of P. dulcis improved activity against
Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella pullorum. | |
dc.publisher | Universidade Federal de Santa Maria | |
dc.publisher | BR | |
dc.publisher | Farmácia | |
dc.publisher | UFSM | |
dc.publisher | Programa de Pós-Graduação em Ciências Farmacêuticas | |
dc.rights | Acesso Aberto | |
dc.subject | Atividade antioxidante | |
dc.subject | Grupos fenólicos | |
dc.subject | DPPH | |
dc.subject | TBARS | |
dc.subject | CLAE | |
dc.subject | Atividade antimicrobiana | |
dc.subject | Antioxidant activity | |
dc.subject | Phenolic groups | |
dc.subject | HPLC | |
dc.subject | Antimicrobial activity | |
dc.title | Estudo comparativo de Glycyrrhiza glabra (alcaçuz) e Periandra dulcis (alcaçuz-da-terra) | |
dc.type | Dissertação | |