In the past decade, the number of urban runners has considerably increased, specially considering its health-related benefits. In Chile, running has been reported as the third most commonly used physical activity (11.9%) after soccer and physical fitness among physically active adults. Motivation, a driving force to achieve personal goals, has been linked to exercise adherence and to the capacity of keeping a healthy life. Many investigations have been conducted to study running motivations across runners through different domains, such as health, personal goals, self-esteem, competition; founding a tendency from internal towards external factors among different experienced runners. Even though running is an easily accessible activity, the knowledge of underlying motivational factors among runners could enhance promotion of physical activity participation, regarding that urban life may reduce an individual’s motivation for pursuing a better physical lifestyle. Therefore, the purpose of this study was to analyse motivations for sustaining a consistent running routine in different runner populations from Santiago de Chile using the Motivations of Marathoners Scales (MOMS-34). This is the first study examining the specific values of motivation in different runner groups in South America.