dc.creator | Diaz, Spthefania | |
dc.creator | Rojas, M | |
dc.creator | Galleguillos, Marco | |
dc.creator | Maturana, C | |
dc.creator | Smith, P | |
dc.creator | Cifuentes, F | |
dc.creator | Contreras, I | |
dc.creator | Smith, P | |
dc.date.accessioned | 2018-01-15T14:44:48Z | |
dc.date.accessioned | 2019-05-17T14:40:09Z | |
dc.date.available | 2018-01-15T14:44:48Z | |
dc.date.available | 2019-05-17T14:40:09Z | |
dc.date.created | 2018-01-15T14:44:48Z | |
dc.date.issued | 2017 | |
dc.identifier | Journal of Fish Diseases, Volume 40, Issue 12 December 2017, Pages 1895–1902 | |
dc.identifier | http://dx.doi.org/10.1111/jfd.12660 | |
dc.identifier | http://hdl.handle.net/11447/1900 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/2674790 | |
dc.description.abstract | To improve the understanding of the piscirickettsiosis pathogenesis, the in vivo apoptosis
modulation of peritoneal macrophages and lymphocytes was studied in juvenile Salmo salar
intraperitoneally injected with Piscirickettsia salmonis. Five fish were sampled at post-exposure days
1, 5, 8 (preclinical), 20 (clinical) and 40 (post-clinical period of the disease), and the leucocytes of
their coelomic washings were analysed by flow cytometry (using the JC-1 cationic dye), TUNEL and
cytology to detect apoptotic cells. A selective and temporal pattern of apoptosis modulation by P.
salmonis infection was observed. Apoptosis in lymphocytes was not affected, whereas it was
inhibited in macrophages but only during the preclinical stage of the induced piscirickettsiosis.
Hence, it is postulated that P. salmonis inhibits macrophage apoptosis at the beginning of the
disease development to survive, multiply and probably be transported inside these phagocytes;
once this process is complete, macrophage apoptosis is no longer inhibited, thus facilitating the exit
of the bacteria from the infected cells for continuing their life cycle. | |
dc.language | en_US | |
dc.publisher | John Wiley & Sons | |
dc.subject | apoptosis | |
dc.subject | fish | |
dc.subject | leukocytes | |
dc.subject | Piscirickettsia | |
dc.subject | salmon | |
dc.title | Apoptosis inhibition of Atlantic salmon (Salmo salar) peritoneal macrophages by Piscirickettsia salmonis | |
dc.type | Artículos de revistas | |