Artículos de revistas
Rare Genome-Wide Copy Number Variation and Expression of Schizophrenia in 22q11.2 Deletion Syndrome
Fecha
2017Registro en:
Am J Psychiatry. 2017 Nov 1;174(11):1054-1063
Autor
Bassett, Anne
Lowther, Chelsea
Merico, Daniele
Costain, Gregory
Chow, Eva
van Amelsvoort, Therese
McDonald-McGinn, Donna
Gur, Raquel
Swillen, Ann
Van den Bree, Marianne
Murphy, Kieran
Gothelf, Doron
Bearden, Carrie
Eliez, Stephan
Kates, Wendy
Philip, Nicole
Sashi, Vandana
Campbell, Linda
Vorstman, Jacob
Cubells, Joseph
Repetto, Gabriela
Simon, Tony
Boot, Erik
Heung, Tracy
Evers, Rens
Vingerhoets, Claudia
van Duin, Esther
Zackai, Elaine
Vergaelen, Elfi
Devriendt, Koen
Vermeesch, Joris
Owen, Michael
Murphy, Clodagh
Michaelovosky, Elena
Kushan, Leila
Schneider, Maude
Fremont, Wanda
Busa, Tiffany
Hooper, Stephen
McCabe, Kathryn
Duijff, Sasja
Isaev, Karin
Pellecchia, Giovanna
Wei, John
Gazzellone, Matthew
Scherer, Stephen
Emanuel, Beverly
Guo, Tingwei
Morrow, Bernice
Marshall, Christian
International 22q11.2DS Brain and Behavior Consortium
Institución
Resumen
OBJECTIVE:
Chromosome 22q11.2 deletion syndrome (22q11.2DS) is associated with a more than 20-fold increased risk for developing schizophrenia. The aim of this study was to identify additional genetic factors (i.e., "second hits") that may contribute to schizophrenia expression.
METHOD:
Through an international consortium, the authors obtained DNA samples from 329 psychiatrically phenotyped subjects with 22q11.2DS. Using a high-resolution microarray platform and established methods to assess copy number variation (CNV), the authors compared the genome-wide burden of rare autosomal CNV, outside of the 22q11.2 deletion region, between two groups: a schizophrenia group and those with no psychotic disorder at age ≥25 years. The authors assessed whether genes overlapped by rare CNVs were overrepresented in functional pathways relevant to schizophrenia.
RESULTS:
Rare CNVs overlapping one or more protein-coding genes revealed significant between-group differences. For rare exonic duplications, six of 19 gene sets tested were enriched in the schizophrenia group; genes associated with abnormal nervous system phenotypes remained significant in a stepwise logistic regression model and showed significant interactions with 22q11.2 deletion region genes in a connectivity analysis. For rare exonic deletions, the schizophrenia group had, on average, more genes overlapped. The additional rare CNVs implicated known (e.g., GRM7, 15q13.3, 16p12.2) and novel schizophrenia risk genes and loci.
CONCLUSIONS:
The results suggest that additional rare CNVs overlapping genes outside of the 22q11.2 deletion region contribute to schizophrenia risk in 22q11.2DS, supporting a multigenic hypothesis for schizophrenia. The findings have implications for understanding expression of psychotic illness and herald the importance of whole-genome sequencing to appreciate the overall genomic architecture of schizophrenia.