dc.creator | Polanco,Víctor [Chile. Universidad Mayor. Centro de Genómica y Bioinformática] | |
dc.creator | Ramírez, Ingrid [Chile. Universidad Técnica Federico Santa María. Centro de Biotecnología Daniel Alkalay Lowitt] | |
dc.creator | Pena-Cortés, Hugo [Chile. Universidad Técnica Federico Santa María. Centro de Biotecnología Daniel Alkalay Lowitt] | |
dc.date.accessioned | 2018-09-07T14:11:42Z | |
dc.date.accessioned | 2019-05-17T14:28:09Z | |
dc.date.available | 2018-09-07T14:11:42Z | |
dc.date.available | 2019-05-17T14:28:09Z | |
dc.date.created | 2018-09-07T14:11:42Z | |
dc.date.issued | 2012 | |
dc.identifier | Díaz Mauricio, Polanco Victor, Ramírez Ingrid, Peña-Cortés Hugo. Molecular cloning and expression analysis of 12-oxophytodienoate reductase cDNA by wounding in Solanum tuberosum. Electron. J. Biotechnol. [Internet]. 2012 Jan [citado 2018 Set 04] ; 15( 1 ): 10-10. Disponível em: https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100010&lng=pt. | |
dc.identifier | ESSN 0717-3458 | |
dc.identifier | https://scielo.conicyt.cl/pdf/ejb/v15n1/a10.pdf | |
dc.identifier | http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v15n1-3/1402 | |
dc.identifier | https://doi.org/10.2225/vol15-issue1-fulltext-3 | |
dc.identifier | http://repositorio.umayor.cl/xmlui/handle/sibum/2738 | |
dc.identifier | 10.2225/vol15-issue1-fulltext-3 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/2672929 | |
dc.description.abstract | Jasmonic acid (JA) and 12-oxophytodienoic acid (OPDA) are signal molecules involved in the stress and defense responses in plants. A full-length cDNA clon of OPR3 encoding 12-oxophytodienoate reductase 3, key enzyme involved in the biosynthesis of JA from linolenic acid was obtained from a Solanum tuberosum cDNA library. Sequence analysis showed that OPR3 encoded a polypeptide of 400 amino acids with a predicted molecular mass of 43.9 kDa and pl of 7.72. The deduced amino acid sequence of OPR3 showed high similarities to other 12-oxophytodienoate reductases. A peroxisomal signal sequence indicates OPR3 probable location in peroxisome. Levels of OPR3 mRNA accumulated in potato leaves reaching maximum levels within 1 hr of mechanical wounding. Elevated levels of JA were correlated to expression of the OPR3 gene. | |
dc.language | en | |
dc.publisher | CIENCIAS | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
dc.subject | BIOTECNOLOGÍA | |
dc.title | Molecular cloning and expression analysis of 12-oxophytodienoate reductase cDNA by wounding in Solanum tuberosum | |
dc.type | Artículos de revistas | |